Serological and molecular biological analysis of RhD--: a case report
Journal Title: Chinese Journal of Blood Transfusion - Year 2023, Vol 36, Issue 12
Abstract
Objective To study the blood group serology and molecular biology of patients with RhD--, so as to guide clinical blood use. Methods The EDTA-K2 anticoagulant blood of the patient was detected for Rh antigens and antibodies. Meanwhile, DNA was extracted, and the 1-10 exon of RHCE and RHAG gene was sequenced by Sanger sequencing. Results The serological test showed O type RhD--, and all spectral cells were positive. RHCE gene sequencing showed RHCE*02/RHCE*02, RHAG gene sequencing showed mutations at site 808 G > A and site 861 G > A of exon 6. Conclusion When patients were with RhD--, and related immune conditions such as pregnancy and/or transfusion history were present, autologous blood transfusion or plasma exchange could be an option for emergency blood use.
Authors and Affiliations
Bo SHUI, Zhibing HOU, Li YAO, Wei YAN, Jiwu HE
Keywords
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- EP ID EP738729
- DOI 10.13303/j.cjbt.issn.1004-549x.2023.12.020
- Views 47
- Downloads 0
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