Serum creatinine: conventional and compensated kinetic method with enzymatic method
Journal Title: International Journal of Clinical Biochemistry and Research - Year 2017, Vol 4, Issue 2
Abstract
Introduction: The overall magnitude and pattern of chronic kidney disease (CKD) in India has been studied sporadically. The estimated glomerular filtration rate (eGFR) is valuable in identifying patients at risk of developing nephropathy. However, calculation of the eGFR from serum creatinine levels is not without limitations. Aim: The aim of this study was to compare analytical performance and workability of the enzymatic method vs kinetic method(both conventional and compensated) for serum creatinine for routine use and to compare the effects of some common interfering substances like bilirubin on the enzymatic method vs kinetic Jaffe’s method. Materials and Method: We measured Serum creatinine by enzymatic, conventional and compensated kinetic creatinine measurement method in 120 samples having bilirubin >1mg/dl. Results: Relationship between Jaff’s kinetic semi-automated method and enzymatic method on Cobas Integra 400 plus for serum creatinine shows regression coefficient 0.88 which means two methods are not correlate with each other. Compensated Jaff’s kinetic method and enzymatic method shows regression coefficient 0.95 which means two methods are correlate with each other and the differences between two methods in Bland-Altman plot shows that compensated Jaff’s kinetic method have negative bias but differences between two methods are less (scatter reading) and constant throughout concentration range. Conclusion: There is good agreement and good comparability of the compensated kinetic Jaffe's method with the enzymatic method than conventional picric acid Jaff’s kinetic method we found that when serum bilirubin was high and serum creatinine was measure with conventional picric acid Jaff’s kinetic method and compensated picric acid Jaff’s kinetic method gives false low creatinine.
Authors and Affiliations
Kiran Chauhan, Dhara Kanani, Priya Patel, N. Haridas
Keywords
Related Articles
- EP ID EP296993
- DOI 10.18231/2394-6377.2017.0048
- Views 72
- Downloads 0
Habitual consumption of Areca catechu may cause increased post prandial blood glucose levels in diabetics: a cross sectional study
Diabetes has become a universal major health concern, personal habits form an integral part of its etiology and poor control of blood sugar is a major contributor of associated morbidity. Betel nut (Areca catechu) has be...
Serum ferritin level in type 2 diabetes mellitus - A case control study
Background: Hyperferritinemia probably contributes insulin resistance and subsequently to decreased insulin secretion causes for development of insulin resistance. Objectives: Compare HbA1c with Ferritin and find correla...
Assessment of Vitamin D status in Type 1 Diabetes Mellitus in pediatric age group
Background: Diabetes mellitus is a group of metabolic disorders characterized by hyperglycemia. Type-1-Diabetes Mellitus (TIDM) results from autoimmune destruction of beta cells leading to insulin deficiency. Vitamin D d...
Role of non-HDL cholesterol in type II diabetes mellitus
Introduction Type 2 diabetes mellitus is a widespread global disorder among which India is the one where 13rd of the population suffering from diabetes and majority of them were diagnosed during the complication Diabetic...
Obesity, obesity related hypertension, their association with adipocytokines among 12-16 years school students
Obesity, obesity related hypertension is increasing at higher rates among adolescents these days. An increase in leptin level and a decrease in adiponectin level were observed in obese individuals with hypertension. Our...