Simple and selective spectrophotometric assay of chloroquine in pharmaceuticals using two nitrophenols as chromogenic agents
Journal Title: IOSR Journal of Pharmacy and Biological Sciences (IOSR-JPBS) - Year 2016, Vol 11, Issue 1
Abstract
Two simple, sensitive and selective spectrophotometric methods are presented for the determination of chloroquine phosphate (CQP) in bulk drug and dosage forms. The methods are based on the interaction of chloroquine (CLQ) with either 2,4-dinitrophenol (DNP) or picric acid (PA) in chloroform resulting in the formation of intensely yellow coloured radical anion which was measured at 430 nm (DNP method) or 420 nm (PA method). The experimental conditions for better performance characteristics of the methods were carefully studied and optimised. Beer’s law is obeyed over the concentration ranges of 3 – 70 and 1.2 – 30 µg mL-1 for DNP method and PA method, respectively, with corresponding molar absorptivity values of 4.7 × 103 and 1.1 × 104 l mol-1 cm -1 . The limits of detection (LOD) and quantification (LOQ) were 1.1 and 3.0 µg mL-1 (DNP method), and 0.3 and 1.2 µg mL-1 (PA method). Intra-day and inter day precisions expressed as relative standard deviation (RSD) were less than 2 and 2.3 %, respectively, whereas, the corresponding accuracies expressed as relative error (%RE) were under 2.5% and 2.3%. The real samples were successfully analysed by using the proposed methods with the results agreeing with the label claim and those of the reference method
Authors and Affiliations
*Basavaiah Kanakapura, Vamsi Krishna Penmatsa1, Chandrashekar Umakanthappa
Keywords
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- EP ID EP383396
- DOI 10.9790/3008-11142940 .
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