SIMPLE HIGH PERFORMANCE LIQUID CHROMATOGRAPHIC METHOD FOR DETERMINATION OF ENANTIOMER OF D-SERINE API.
Journal Title: International Journal of Advanced Research (IJAR) - Year 2019, Vol 7, Issue 8
Abstract
Several amino acids have a chiral center and thus can exist as D- and L-isomers. Amino acid synthesis like D-Serine, L-Serine is use as starting material therefore unreacted L-Serine may present in sample. This L-Serine is an optical enantiomer of D-serine hence this enantiomer need to be quantified. Thus author has developed simple, sensitive, precise, and specific HPLC method for the separation of L-enantiomer of D- Serine using Agilent zorbax SB-C18, 150 mm x 4.6 mm, 3.5 μm HPLC column. The mobile phase used as 10 mMTris buffer with pH 3.0 + 0.05 and Methanol with gradient ratio. D-Serine structure there was no any chromophore are observed therefore author was derivatised the sample with the Marfey?s reagent and further validated the method as per ICH Q2 (R1) guideline and same method can be used for routine analysis.
Authors and Affiliations
Rajendra Phadke , Amit Gosar, Narendra Aarkashi , Deepesh Patil And Rupali Mali.
Keywords
Related Articles
- EP ID EP634272
- DOI 10.21474/IJAR01/9602
- Views 48
- Downloads 0
THE STRATEGY MODEL OF ALTERNATIVE STATE DEFENSE SYSTEM FOR FACING WAR IN DIGITAL ERA.
The purposes of this study is arrage alternative strategies for national defense systems that can be applied today to deal with information warfare in the digital era. This research was conducted at the Office of the Com...
L INTERET DE LA TOMOGRAPHIE PAR COHERENCE OPTIQUE DANS LA PRISE EN CHARGE DE LA MACULOPATHIE DIABETIQUE : ETUDE RETROSPECTIVE A PROPOS DE 100 PATIENTS.
International Journal of Advanced Research (IJAR)
EFFECT OF BONE MARROW DERIVED MESENCHYMAL STEM CELLS VS ALENDRONATE SODIUM IN TREATMENT OF INDUCED ESTROGEN DEFICIENCY OSTEOPOROSIS IN ALVEOLAR BONE OF ALBINO RATS.
Background: Despite extensive efforts for the understanding and curative options for osteoporosis, it remains a critical public health threat. Alendronate sodium, an amino bisphosphonate, is a potent antiresorptive drug...
A PERSPECTIVE:THE USE OF GUIDED IMAGERY AND DRAWINGS AS AN ALTERNATIVE TREATMENT FOR CHILDREN OF TRAUMA.
Cognitive behavioral therapy and pharmaceuticals have traditionally been used to treat trauma-related anxiety in children. Imagery Rehearsal Therapy and meditative breathing with guided imagery have been used as alternat...
DEVELOPMENT OF ADAPTIVE ALGORITHM FOR SPARSE SYSTEM IDENTIFICATION.
In this paper, we develop the adaptive algorithm for system identification where the model is sparse. The classical configurations of adaptive filtering are system identification, prediction, noise cancellation. The low-...