Simultaneous determination of artificial sweeteners in possible counterfeited wines, using high performance liquid chromatography with DAD detection
Journal Title: Ovidius University Annals of Chemistry - Analele Universităţii "Ovidius" Constanţa. Seria chimie - Year 2012, Vol 23, Issue 1
Abstract
Increased use of food, beverages or drugs containing synthetic sweeteners presents a real danger to health, which is why the EU Member States had to establish a system of regular surveys to monitor sweetener consumption. In case of wine industry, according to law no. 244/2002, Romania prohibits the addition of synthetic sweeteners in wine in order to obtain sweet wine. The official method for detection of adulterated sweet wines with synthetic sweeteners is TLC-Thin Layer Chromatography. However, quantitative methods of analysis are needed to measure levels of sweeteners in different food matrices and high performance liquid chromatography has proved to be a powerful tool for quantitative analysis of compounds at traces levels. In this paper, a high performance liquid chromatographic (HPLC) method for the simultaneous separation and determination of three of the most popular artificial sweeteners (acesulfame potassium, saccharine and aspartame) in a single injection was developed. The described method is rapid, accurate and highly sensitive. Detection limit were 4 mg/L for acesulfame K, 1 mg/L for saccharine and 9 mg/L for aspartame respectively. The precision of the method was about 2% and recovery ranged between 92.6% and 103.3%. There were analyzed commercial wine samples, in order to detect possible counterfeits-sweet and medium sweet wines. Therefore, of the 20 analyzed wine samples, only two samples consisting in wine sweet table, were counterfeited by adding saccharin.
Authors and Affiliations
GEANA Elisabeta-Irina, IORDACHE Andreea-Maria, IONETE Roxana Elena
Keywords
Related Articles
- EP ID EP98673
- DOI -
- Views 98
- Downloads 0
Environmental pollution in the petroleum refining industry
The petroleum refining industry has a significant influence on the total pollution of the environment by industrial discharges and wastes. In the operation of petroleum refineries, the atmosphere is polluted with hydroge...
Retinol, cholecalciferol and alpha-tocopherol contents of Bulgarian Black Sea fish species
The aim of the present study is to determine and to compare the content of retinol, cholecalciferol and alpha-tocopherol in edible tissue of two Black sea fishes - Garfish (Belone belone) and Turbot (Psetta maxima). All-...
Spectrophotometric determination of ascorbic acid in grapes with the Prussian Blue reaction
The objective of the present work was to adapt the Prussian Blue reaction for the determination of ascorbic acid. The procedure was successfully applied for the determination of ascorbic acid in red and white grapes ([i]...
Nitrite determination in spices
The aim of this study is to determine the nitrite content in some spices: basil, pepper, dill, thyme, oregano, bay leaves, cinnamon, mint, mustard and cumin. The nitrite content was measured using UV-VIS spectrometric m...
Inorganic fuels mixtures for automotives propulsion engines
The objective of this study is the evaluation of the opportunity to use an inorganic oxygenated compound as ammonium nitrate or urea, to reduce fossil fuels pollution. Our research takes into account the ammonium nitrate...