Simultaneous Determination of Lamivudine and Tenofovir Disproxil Fumarate by UV Spectrophotometric Method
Journal Title: Journal of Medical Science And clinical Research - Year 2015, Vol 3, Issue 4
Abstract
A simple and rapid UV spectrophotometric method has been developed for simultaneous estimation of Lamivudine (LAM) and Tenofovir disoproxil fumarate (TDF). The absorption maxima of both drugs were found at 268nm and 258nm and obeyed Beer’s law in the range of 2-10μg/ml (y = 0.059x-0.02; r2 = 0.998) and 2-10μg/ml (y = 0.0565x-0.017; r2 = 0.997) respectively for LAM and TDF in water: water (50:50) solvent system. Accuracy and reproducibility of the proposed method was statistically validated by recovery studies. This method is found to be precise and accurate and can easily be employed in the laboratory for the routine estimation of drugs.
Authors and Affiliations
Ashwini S. Mali
Keywords
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