Simultaneous Determination of Three Different Alkaloids: Scopolamine, Hyoscyamine and Lobeline in Pharmaceutical Dosage Forms by UFLC Method
Journal Title: IOSR Journal of Pharmacy and Biological Sciences (IOSR-JPBS) - Year 2017, Vol 12, Issue 5
Abstract
A rapid and accurate UFLC method was developed for determination of scopolamine, hyoscyamine and lobeline in herbal cough mixture found in herbal product (BN) purchased from local market. Reversed phase chromatographic analyses was carried out under gradient conditions using column ACE, C18 (150 x 4.6 mm); packed with 5 µm diameter particles. The mobile phase was Acetonitrile (A) and (buffer was composed 0.1% phosphoric acid and 0.1% sodium lauryl sulfate in distilled water) (B). The gradient mobile phase was as follows: 35% (A): 65% (B) for 10 min; then 80% (A): 20% (B) for 12 min; then 35% (A): 65% (B) for 5 min. The flow rate was 2.0 ml/min. The injection volume was 20 µl. The detection wavelength (λ max) was 205 nm using a photodiode array detector. Linearity of the method was established over the concentration ranges of 80 – 500 µg.ml -1 for scopolamine hydrobromide trihydrate with a retention time about 5.77 minutes, 18 – 112.5 µg.ml -1 for hyoscyamine sulfate with a retention time about 7.52 minutes and 20 – 125 µg.ml -1 for lobeline sulfate with a retention time about 17.74 minutes. Correlation coefficients was greater than 0.99. The recovery level of scopolamine hydrobromide trihydrate, hyoscyamine sulfate and lobeline sulfate were 100.02%, 100.02% and 99.96%; respectively. The method described is quite suitable for routine analysis of herbal extracts and in pharmaceutical preparations.
Authors and Affiliations
*Mohamed A. Mahmoud, Mokhtar M. Bishr 1, , Mohamed A. El-Degwy 1, Mohamed A. Amin 1
Keywords
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- EP ID EP391217
- DOI 10.9790/3008-1205054652
- Views 84
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