Simultaneous Estimation of Abacavir Sulfate and Lamivudine in their Combined Dosage Form by First Derivative Zero Crossing and Ratio First Derivative Spectroscopic Methods
Journal Title: Indo American Journal of Pharmaceutical Research - Year 2011, Vol 1, Issue 5
Abstract
Two simple, sensitive, specific and accurate spectrophotometric methods are proposed in the present work for the simultaneous estimation of Abacavir sulfate (ABA) and Lamivudine (LMV) in their binary mixture. In first derivative zero crossing technique the absorbances at 234.2 nm (zero crossing point of LMV) were plotted against the respective concentrations of ABA. Similarly the absorbances at 287.3 nm (zero crossing point of ABA) were plotted against the respective concentrations of LMV. For the ratio spectra first derivative spectrophotometric method, the graphical treatment of the overlapping spectra depends on division of the absorption spectrum of the binary mixture by a standard spectrum of one of the components and then the first derivative amplitudes measured at 233.0nm for ABA and 236.4nm for LMV .The calibration curves follow Beer’s law in the range of 5 to 30 μg/ml for ABA and 2.5 to 15 μg/ml LMV for both the methods. The methods were successfully applied to quantify ABA combined with LMV in tablets and validated accordingly.
Authors and Affiliations
DIPTI RUIKAR, DITI DESAI, BANSARI MYANGAR, SADHANA RAJPUT
Keywords
Related Articles
- EP ID EP221651
- DOI -
- Views 177
- Downloads 0
FORMULATION OF CO-CRYSTAL FOR SOLUBILITY ENHANCEMENT OF CURCUMIN
Abstract Co-crystallization holds a great potential for solving the problem of low aqueous solubility and bioavailability of drug molecules and improve the other physical properties of active pharmaceutical ingredients....
ROLE OF HYDROPHILIC POLYMERS ON THE STABILITY OF FORMULATIONS OF TENOXICAM – SBE7-β-CD INCLUSION COMPLEX
The present study was carried out to evaluate the usefulness of sulfobutyl ether β-Cyclodextrin (SBE7-β-CD) in increasing the dissolution rate of tenoxicam. The approach employed to prepare the inclusion complexes of the...
STUDIES ON Α-AMYLASE INHIBITOR ISOLATED FROM ADIANTUM AETHIOPICUM L.
Abstract Fresh fronds of Adiantum aethiopicum L were collected from Fergusson College campus at various growth stages like young fronds, mature fronds without sori, mature fronds with immature sori (pale green colour) an...
“AN INTERVENTIONAL STUDY ON KNOWLEDGE, ATTITUDE & PRACTICE TOWARDS URINARY TRACT INFECTION AMONG ADOLESCENT GIRLS STUDENTS IN SELECTED GIRLS SCHOOLS IN CHITRADURGA CITY”
Background: Urinary tract infection (UTI) is a bacterial infection found to be more prevalent among the adolescent girls than any other age groups. Therefore, a good promotional dissemination on UTI is important to conve...
AN IMPACT OF CLINICAL PHARMACIST IN IMPROVING MEDICATION ADHERENCE AND QUALITY OF LIFE OF PATIENTS IN RURAL AREA
Abstract The patient-centered medication management program by the clinical pharmacist has been characterized as a model of health care delivery that improves the patient care. These services are intended to be continuou...