SIMULTANEOUS ESTIMATION OF LOPERAMIDE HYDROCHLORIDE AND TINIDAZOLE IN BULK AND FORMULATIONS BY REVERSE PHASE HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY
Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2016, Vol 8, Issue 9
Abstract
Objective: A new, rapid, selective, precise, accurate and economical, isocratic, reverse phase high-performance liquid chromatography method has been developed for simultaneous estimation of loperamide hydrochloride and tinidazole in bulk and in tablet formulations.Methods: The separation was achieved by using Lithosphere RP C-18, (250 x 4.6 mm, 5 µm) end capped column with a mobile phase containing sodium-1-octane sulfonate buffer: methanol: acetonitrile (40:30:30%v/v/v) pH adjusted to 4.0 (using dilute orthophosphoric acid). The flow rate was 1.0 ml/m and column effluent was monitored at 224 nm. The method was validated as per international conference on chemical harmonization (ICH) guidelines.Results: Tinidazole and loperamide hydrochloride were eluted at about 3.1 and 5.4 min respectively, indicating the shorter analysis time. The proposed method was found to be accurate, precise and reproducible. The linearity was established in the concentration range of 10-50 µg/ml. Limit of detection (LOD) and Limit of quantification (LOQ) was found to be 0.001 µg/ml and 0.003 µg/ml for loperamide hydrochloride and 0.01 µg/ml and 0.03 µg/ml for tinidazole.Conclusion: This method can be used for routine analysis of formulations containing any of the above drugs or combinations without any alteration in the chromatographic conditions.
Authors and Affiliations
Poornima K. , Channabasavaraj Kp.
Keywords
Related Articles
- EP ID EP576029
- DOI 10.22159/ijpps.2016.v8i9.12864
- Views 106
- Downloads 0
STABILITY INDICATING RP-HPLC-PDA METHOD FOR DETERMINATION OF ABIRATERONE ACETATE AND CHARACTERIZATION OF ITS BASE CATALYZED DEGRADATION PRODUCT BY LC-MS
Objective: The present work describes stability indicating (SI) RP-HPLC-PDA method for determination of abiraterone acetate (ABA) and characterization of its base catalyzed degradation product by LC-MS.Methods: The separ...
INCIDENCE AND RISK FACTORS OF RENAL IMPAIRMENT IN HIV-1 INFECTED PATIENTS RECEIVING TENOFOVIR BASED ANTIRETROVIRAL THERAPY IN A SOUTH INDIAN HOSPITAL
Objective: To identify the incidence and risk factors of tenofovir (TDF) induced nephrotoxicity among People Living with HIV/AIDS (PLHA) receiving TDF-based anti-retroviral therapy (ART) in a South Indian Hospital.Method...
DESIGN OF A FUZZY MODEL FOR THALASSEMIA DISEASE DIAGNOSIS: USING MAMDANI TYPE FUZZY INFERENCE SYSTEM (FIS)
Objective: Diagnosis process of Thalassemia requires several types of medical test, and results of this test together identify the stage of Thalassemia. The objective of this study is to design a Fuzzy Inference System t...
IN VITRO AND IN VIVO ANTIHYPERGLYCEMIC EFFECT OF ACTIVE FRACTION OF CLEOME RUTIDOSPERMA DC
Objective: The present study was aimed to investigate the possible mechanism of action of the active sub fraction of C. rutidosperma. This was done by in vivo and in vitro method.Methods: Aqueous extract of C. rutidosper...
IN VITRO ANTIBACTERIAL ACTIVITY OF SELECTED UNDERUTILIZED PLANTS AND CYTOTOXIC PROPERTY OF TERMINALIA CATAPPA
Objective: This study aimed at determining the antibacterial activity of the underutilized plants of Sri Lanka, “Kottamba†(Terminalia catappa), “Purpurata†(Alpinia purpurata) and “Harankaha†(Curcuma zedoar...