SIMULTANEOUS RP-HPLC ANALYSIS OF QUERCETIN AND KAEMPFEROL IN DIFFERENT PLANT PARTS OF CISSUS QUADRANGULARIS
Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2016, Vol 8, Issue 7
Abstract
Objective: To develop a simple, rapid and specific reversed phase High-Performance Liquid Chromatography (HPLC) method for comparative analysis of flavonoids, quercetin and kaempferol, in different plant parts (leaves, stem and roots) of Cissus quadrangularis Linn.Methods: The HPLC method which can be used effectively for separation of components from plants has been developed to perform a comparative analysis of flavonoids, quercetin and kaempferol, in different plant parts (leaves, stem and roots) of Cissus quadrangularis Linn. An endcapped C18 column at 370 nm, and water: acetonitrile (45:55) containing 0.1% o-phosphoric acid as mobile phase was used.Results: Quercetin and kaempferol were well resolved at about 5 min and 7 min respectively. Calibration curves for quercetin and kaempferol were linear in the range of 1-10 µg/ml (R2= 0.999) and 0.5-10 µg/ml (R2= 0.999) respectively. The sensitivity of the method was found to be higher, with a limit of detection (LOD) values of 0.42 μg/ml for kaempferol and 0.48 μg/ml for quercetin and limit of quantification (LOQ) values for kaempferol and quercetin 1.27 μg/ml and 1.47 μg/ml respectively. Percentage recovery values for kaempferol were found to be 99.18, 99.03 and 98.32 for the leaves, stem and root respectively, and for quercetin, they were 99.77, 100.12 and 100.54 for the leaves, stem and root respectively.Conclusion: The developed HPLC method for the analysis of flavonoids has enabled rapid, accurate and reproducible determination. The method can be applied successfully for analysis of quercetin and kaempferol in various plant parts of C. quadrangularis.Keywords: Cissus quadrangularis, HPLC, Kaempferol, Quercetin
Authors and Affiliations
Vandana Jain, Mohammed Shadab Shaikh
Keywords
Related Articles
- EP ID EP576225
- DOI -
- Views 86
- Downloads 0
UV SPECTROPHOTOMETRIC DETERMINATION OF RUPATADINE FUMARATE IN BULK AND TABLET DOSAGE FORM BY USING SINGLE POINT STANDARDIZATION METHOD
Objective: To develop and validate a simple, selective, precise and accurate method for the estimation of rupatadine fumarate in bulk and tablet dosage form by using the single point standardization method as per interna...
PREPARATION AND CHARACTERIZATION OF GEMCITABINE LOADED MPEG-PCL POLYMERIC NANOPARTICLES FOR IMPROVED TRANSPORTATION ACROSS BLOOD BRAIN BARRIER
Objective: To prepare Gemcitabine (GCB) loaded Methoxy Polyethylene Glycol-Poly (Caprolactone), (MPEG-PCL) nanoformulations and to carry out the physicochemical characterisation with a primary objective to enhance the tr...
GENOTYPE AND ABO BLOOD GROUP ASSOCIATION WITH PREVALENCE OF MALARIA AMONG PATIENTS IN UNIVERSITY OF NIGERIA MEDICAL CENTER: A CROSS-SECTIONAL EVALUATION
Objective: The aim of this study was to evaluate the prevalence of malaria and its relationship with the ABO blood group and genotype at the University of Nigeria Medical Centre. Methods: The study had a prospective cro...
PHYTOPHENOLICS COMPOSITION, HYPOLIPIDEMIC, HYPOGLYCEMIC AND ANTIOXIDATIVE EFFECTS OF THE LEAVES OF FORTUNELLA JAPONICA (THUNB.) SWINGLE
Objective: Fortunella japonica (Thunb.) Swingle is an evergreen shrub, its whole fruit, including the peel, is eaten. There have been few detailed phytophenolics composition reports on this genus and the hypoglycemic and...
SYNTHESIS, CHARACTERIZATION AND ANTI INFLAMMATORY ACTIVITY OF NOVEL QUINOXALINE DERIVED CHALCONES
Objective: Quinoxaline derivatives were reported with wide range of biological activities. Hence it was planned to synthesize and screen for their anti inflammatory (in vivo) activity.Methods: Orthophenylene diamine was...