SIMULTANEOUS SPECTROPHOTOMETRIC DETERMINATION OF FEBUXOSTAT AND ALLOPURINOL IN SYNTHETIC MIXTURE
Journal Title: International Research Journal of Pharmacy (IRJP) - Year 2016, Vol 7, Issue 4
Abstract
The present manuscripts describe simple, sensitive, rapid, accurate, precise and economical absorbance ratio method for the simultaneous determination of Febuxostat and Allopurinol in synthetic mixture. Absorbance ratio method uses the ratio of absorbances at two selected wavelengths, one which is an isoabsorptive point and other being the λ-max of one of the two components. Febuxostat and Allopurinol show an isoabsorptive point at 274 nm in methanol. The second wavelength used is 250 nm, which is the λ-max of Allopurinol in methanol. The linearity was obtained in the concentration range of 2-12 μg/ml for both Febuxostat and Allopurinol. The concentrations of the drugs were determined by using ratio of absorbances at isoabsorptive point and at the λ-max of Allopurinol. The method was successfully applied to synthetic mixture because no interference from the mixture excipients was found. The results of analysis have been validated statistically and by recovery studies according to ICH guidelines.
Authors and Affiliations
Satish Patel , Maulik Patel
Keywords
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- EP ID EP106979
- DOI 10.7897/2230-8407.07438
- Views 103
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