SINGLE STRAND CONFORMATION POLYMORPHISM (SSCP) OF BACILLUS SPECIES USING REC A CANDIDATE GENE APPROACH
Journal Title: International Journal of Agriculture Sciences - Year 2016, Vol 8, Issue 17
Abstract
The aim of the study was to isolate and characterize Bacillus species, which could be used as probiotic candidates. Initially, Bacillus isolates were collected from milk, cream, fresh tomato and soil. Based on morphological and biochemical tests, 37 Bacillus isolates including 3 standard cultures were subjected to molecular characterization using polymerase chain reaction (PCR) single strand conformation polymorphism (SSCP) technique with partial recA gene. The SSCP analysis of 263 bp partial recA amplicons of Bacillus isolates yielded complex SSCP patterns on 8% polyacrylamide gel. On the basis of SSCP band pattern of partial recA PCR products, all the selected isolates were classified into twelve different groups. The SSCP analysis of partial recA PCR products was found to be a powerful tool for illustrating polymorphism or heterogeneity within Bacillus species.
Authors and Affiliations
LOPAMUDRA HALDAR
Keywords
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