Somatic embryo formation in Persea americana Mill var. Catalina from immature zygotic embryos
Journal Title: Biotecnología Vegetal - Year 2005, Vol 5, Issue 2
Abstract
The establishment of embryogenic culture of avocado have been achieved in different genotypes, usually the immature zygotic embryos are the initial explants and the process has been described in several variety. In the present paper the induction of the somatic embryogenesis in avocado (Catalina variety) from zygotic embryos is proposed. Zygotic embryos taken from unripe fruits were used as explants . The fruits were divided into five groups according to their size. The embryos were cultured in a medium containing 4-amino-3,5,6 trichlorpicolinic acid (Picloram) in concentrations of 0.1, 0.4, and 0.6 uM. The culture medium used for the induction of the somatic embryogenesis consisted of: Macro B5, Micro MS, thiamine (0.8 mg.l -1 ), myo-inositol (100 mg.l -1 ), sucrose (30g.l -1 ) and pH 5.7. The number of zygotic embryos with opened cotyledonal leaves was evaluated starting from the third day of culture. It was also evaluated the number of fenolized zygotic embryos at the third week of culture and the presence of somatic embryos five weeks after the culture initiation. The formation of somatic embryos was achieved in all the treatments. The highest number of explants that formed somatic embryos was achieved when a concentration of 0.6 uM of Picloram was used and the second group of size (0.71 x 0.65 mm) observing significant differences between the different groups of fruit size.
Authors and Affiliations
Lillien Fajardo Rosabal , Marisol Freire Seijo , Yudith García Rodríguez , Leandris Argentel Martínez
Keywords
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