Specific Single Chain Antibodies (Scfvs) Against Influenza a Virus Using Phage Display Technique
Journal Title: Journal of Immunology and Vaccine Technology - Year 2014, Vol 1, Issue 1
Abstract
Introduction: 2009 H1N1 swine-origin Influenza A virus with human pandemic potential emerged in 2009 and caused a number of mortality. Two highly conserved regions 173-181 and 227-239 has recognized in Hemagglutinin of H1N1 Influenza irus. Immunity to the different subtypes of Influenza virus could obtain with high affinity antibodies against conserved pitopes of the virus. In the present study, a phage antibody display library of scFv was applied for the selection of scFv ntibodies against the conserved sequences of the hemagglutinin. Materials and Methods: Panning process was used to solate specific scFvs against highly conserved neutralizing epitopes of Hemagglutinin. PCR and fingerprinting were done on he selected clones. Phage ELISA was performed to evaluate the specificity of the clones selected against the epitopes. Results: Two common patterns gainst each peptide were obtained which represented the isolation of two specific scFvs, cFv- and svFv-II with the frequencies of 25% (5/20) and 55% (11/20) against peptide 1 and scFv-I’ and scFv- II’ with the frequencies f 20% (4/20) and 75% (15/20) against peptide 2. Phage ELISA showed the reactivity of the scFv antibodies with the related pitopes while no reaction with unrelated epitopes and other negative controls were obtained. Conclusion: Results emonstrated the isolation of two specific scFvs against two conserved neutralizing sequences of Hemagglutinin of Influenza virus A. Due to several advantages of scFv, these antibodies are intro
Authors and Affiliations
Foroogh Nejatollahi
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