Spore Forming Bacterium from Oil Contaminated Soil as a Source of a Lipase Enzyme with Exogeneous Lipolytic Activity
Journal Title: IOSR Journal of Pharmacy and Biological Sciences (IOSR-JPBS) - Year 2017, Vol 12, Issue 1
Abstract
Twenty two bacterial isolates were obtained from oil contaminated soil, collected from some oil stations in Jeddah. All the obtained bacterial isolates were screened on Tween-Yeast extract medium for lipase production. Three bacterial isolates HM10, HM15 and HM20 showed the highest growth and lipase production agar medium, thus they were grown in liquid olive oil medium at 120 rpm. Maximum lipase production was obtained by the isolate HM10. The isolate HM10 was characterized and identified through physiological, biochemical tests and culture characteristics in addition to 16S rDNA as Bacillus coagulans. The effects of different factors on the enzyme production were studied. It was found that bacterial growth in medium 4 at initial pH 7.0, containing olive oil and incubation at 37ºC for 2 days at 120 rpm were the most favorable conditions for maximum lipase production by the tested isolate. The bacterial isolate was grown using the best culture conditions and lipase was precipitated using 80% of ammonium sulphate, purified using colum chromatography and characterized. The molecular weight was 62 kDa and the maximum enzyme activity was at 50ºC and pH 7.0. Presence of K + and Ca++ ions enhance enzyme activity.
Authors and Affiliations
Magda M. Alya* ,, Hadeel Abdul-Aziz Al-Shareef, Huda A. Quria, Madeha N. Al-seeni b
Keywords
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- EP ID EP387265
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