Stability and Optimal Lipolytic Activity of Sonicatedprunus Avium Lipase and its Application as Fat Degradation and Hydrolysis of Poultry wastewater
Journal Title: International Journal of Applied and Natural Sciences - Year 2018, Vol 7, Issue 6
Abstract
Present work is an optimized lipolytic activity of sonicated defatted Prunus Avium seed lipases. The characterization and the stability of P. avium lipase were assayed at incremental steps of pH and temperature. Comparison of fat liberation potential of the Lipases treated and untreated poultry wastewater and mutton fat showed the importance of Avium lipase towards fat hydrolysis. A 300W sonicated waves in 30 minutes made meal clear for enzyme extraction by removing all interference of seeds fat, evidenced by the images of scanning electron microscope.10 % olive oil emulsion ; a substrate, one-hour incubation time and 4000rpm agitation speed were the optimal variables for extraction of enzyme amongst the 9 runs of experiments suggested by orthogonal L9 array design. Substrate concentration was found the actual contributor to facilitating the lipase for complete hydrolysis. A 43.19%contribution (ANOVA result) of olive oil: substrate was found responsible for maximum lipolytic activity (1.52μU) of Avium lipase at pH 6 and 400C.Best stability of lipase was observed in n-heptane. A release of 6 times more poultry wastewater fat as compared to acid hydrolysis was the successful application of P. avium after 180minutes of the incubation period. Degradation of lipids as increase the %age of monounsaturated acid as compared to polyunsaturated acid (GLC analysis) speculating that P. avium enzyme can be utilized in oil/ fat, detergent and wastewater industry due to fat degradation property.
Authors and Affiliations
Hifza Akhtar, Muhammad Ilyas Tariq, Nasrgis Sultana, Ambreen Imtiaz
Keywords
Related Articles
- EP ID EP410443
- DOI -
- Views 103
- Downloads 0
NOVEL BITTER MELON (MOMORDICA CHARANTIA L.) AND OLIVE LEAVES (OLEA EUROPAEA L.) PHYTOSOMES: PREPARATION AND ITS EVALUATION FOR ANTI-HYPERGLYCEMIC ACTIVITIES BY ORAL GLUCOSE TOLERANCE TEST (OGTT)
Objectives The objectives of this study was to prepare and investigate the phytosomes of crude bitter melon and olive leaves extracts for anti-hyperglycemic activities in glucose-induced hyperglycemic rats. Methods Phyto...
STUDY OF ELECTRICAL PROPERTIES OF CADMIUM SULPHIDE THIN FILMS WITH POLYANILINE FOR OPTOELECTRONIC APPLICATIONS
A thin film of CdS and Polyaniline has been developed by vacuum evaporation technique for their electrical characterization in this paper. These prepared thin films then deposited onto glass as well as the metallic subst...
DRY SEASON ECOLOGY AND SPECIES COMPOSITION OF MOSQUITO’S FAUNA IN WHITE NILE AREA CENTRAL SUDAN
This study carries out in Ed Dueim town, White Nile State Sudan from April, to June 2011 to update mosquito species, their ecology, and the resting places. Three methods adopted for mosquito survey: standard dipper for t...
A New Hexahydroxysterol from a Soft Coral Sarcophyton Elegance Moser from the Coasts of Gulf of Mannar of Indian Ocean
A new hexahydroxysterol, (24S) -24-methylcholestane-1β, 3β, 5α, 6β ,25, 26-hexol 25-monoacetate (5) was isolated from a soft coral Sarcophyton elegance Moser collected from the coasts of the Gulf of Manner of Indian Ocea...
Impact of MGNREGA to Achieve Food Security among Rural Household
Today, India has a population of over 1.21 billion, of which, 250 million people are below the poverty line and approximately 75 percent of them reside in rural areas, making the employment of rural people as crucial for...