Stability indicating assay method for simultaneous estimation of nebivolol and valsartan in pharmaceutical dosage form by RP-HPLC

Journal Title: Asian Journal of Green Chemistry - Year 2018, Vol 2, Issue 3

Abstract

A simple, rapid, precise, and accurate isocratic reversed-phase stability indicating HPLC method was developed and validated for simultaneous determination of the nebivolol and valsartan in the tablet dosage form. The effective chromatographic separation was achieved by a YMC pack pro octadecyl silane (150×4.6 mm, 3 µm) column using a mobile phase composed of methanol: acetonitrile: 0.05 M potassium dihydrogen phosphate buffer (Ph=3.0 with 10% ortho phosphoric acid after addition of 0.2% triethylamine) (30:30:40, v/v/v) at a flow rate of 1 mL/min and UV detection at 282 nm. Drugs were subjected to the acid, base, oxidation, heat, and photolysis to apply the stress. Linearity ranges were 5–30 µg/mL (r2= 0.9989) for nebivolol and 80-480 µg/mL (r2=0.9991) for valsartan. Limit of detection was 0.38 µg/mL and 1.08 µg/mL for nebivolol and valsartan, respectively. The limit of quantification for the nebivolol and valsartan was 1.15 µg/mL and 3.27 µg/mL, respectively.

Authors and Affiliations

Amit Vyas, Grishma Nathwani, Ajay Patel, Ashok Patel, Nilesh Patel, Nirav Makvana

Keywords

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  • EP ID EP468773
  • DOI 10.22034/ajgc.2018.61081
  • Views 94
  • Downloads 0

How To Cite

Amit Vyas, Grishma Nathwani, Ajay Patel, Ashok Patel, Nilesh Patel, Nirav Makvana (2018). Stability indicating assay method for simultaneous estimation of nebivolol and valsartan in pharmaceutical dosage form by RP-HPLC. Asian Journal of Green Chemistry, 2(3), 227-245. https://europub.co.uk./articles/-A-468773