STABILITY INDICATING METHOD DEVELOPMENT AND VALIDATION FOR THE QUANTIFICATION OF ISOPROTERENOL HCL IN BULK AND ITS FORMULATION BY RP-HPLC USING PDA DETECTION
Journal Title: Journal of Pharmaceutical and Scientific Innovation - Year 2018, Vol 7, Issue 5
Abstract
Theobjective of the present study was to develop an accurate, precise stability indicating reverse phase liquid chromatographic method to quantify Isoproterenol HCl in bulk and its formulation using PDA detection. The chromatographic separation of the analyte from the degradants was achieved on Phenomenex Luna columnwith mobile phase composition of Methanol and 0.1% Triethyl amine [pH 7.0], [20:80% v/v]at flow rate of 1.0 ml/min. The analyteseparation was monitored using PDA detection at 279nm. The method was linear in the concentration range of 10-60μg/ml.The method shown acceptablepercent relative deviations for the Inter-day (0.58%) and Intra-day (0.65%) precisions. The mean percent recovery for accuracy study was within the limit. From the stability studies method has proven specificity to quantify Isoproterenol HCl in presence of its degradation products.The method shown acceptance as according to ICH guidelines.
Authors and Affiliations
S. Muneer, Hindustan Abdul Ahad, Kothapalli Bonnoth Chandrasekhar
Keywords
Related Articles
- EP ID EP631503
- DOI 10.7897/2277-4572.075105
- Views 71
- Downloads 0
ASSESSING THE BEST POLY VINYL PYRROLIDONE AS A CARRIER FOR ETORICOXIB SOLID DISPERSIONS: FABRICATION AND EVALUATION
This present search is to find the best Poly Vinyl Pyrrolidone as a carrier for preparing solid dispersions by taking Etoricoxib as a model drug. Five varieties of Poly Vinyl Pyrrolidone i.e., PVP K-12, PVP K-17, PVP K-2...
EMERGING APPLICATIONS OF FERMENTED FRUIT ENZYME BEVERAGES AS NUTRACEUTICAL, FUNCTIONAL FOOD, AND DESIGNER FOOD PRODUCTS: A REVIEW
In the past, the beneficial effects of fermented fruits were unknown, and so people primarily used fermentation to preserve fruits, enhance shelf life, and improve flavor. Fermentation of fruits has been adopted over man...
TECHNICAL SHEET FOR IMPROVING POST HARVEST STORAGE AND CONSERVATION OF KOLANUTS
Kolanut is one of the most popular fruits in traditional ceremonies in Africa and it has great added value when it is well preserved for a long time. However, we found lot of losses during storage due to handlingand poor...
POLYMERASE CHAIN REACTION AND ITS CLINICAL APPLICATION: A REVIEW
Polymerase chain reaction is a popular method in molecular diagnostic in which small amount of DNA undergo five steps of denaturation, primer attachment, again repeating to form number of copies, in analogous way continu...
FORMULATION AND EVALUATION OF FAST DISSOLVING BUCCAL FILM OF ENALAPRIL MALEATE
In the development of buccal drug delivery system, mucoadhesion i.e.; the binding to mucin layer of biological membrane is the key element. Buccal film delivery system consist of at thin film, which is simply placed on t...