STABILITY INDICATING RP HPLC METHOD FOR THE ESTIMATION OF ARMODAFINIL IN TABLET DOSAGE FORM
Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2014, Vol 6, Issue 8
Abstract
Objective: To develop simple reverse phase HPLC method for the estimation of Armodafinil in tablet dosage form.Methods: Chromatography was performed by isocratic elution on a Stainless steel Hibar C18 column with dimensions 4.6 x 250 mm, packed with octadecylsilane bonded to porous silica (C18) with particle size 5 micron. Acetonitrile and water in the ratio of 50:50 v/v is used as mobile phase. The flow rate is 1.0 ml/ min and effluent is monitored at 220 nm. Armodafinil was eluted at a retention time of 3.8 minutes.Results: The standard curve of Armodafinil was linear over a working range of 1–700 µg/ml and gave an average correlation coefficient of 0.999. The limit of quantitation (LOQ) of the drug is 0.1 µg/ ml. Recovery studies were carried out by standard addition method and the recoveries are found satisfactory within the range of 99.3 to 101.5 %. The method is precise with % RSD belowConclusion: The method is validated in terms of robustness and forced degradation studies were carried out and this method can be applied for routine degradation studies ans quantification in regular laboratories.
Authors and Affiliations
P Vivek Sagar, Nelofer Bagum, S. Shobha Rani
Keywords
Related Articles
- EP ID EP579781
- DOI -
- Views 102
- Downloads 0
FORMULATION OF KETOCONAZOLE LOADED NANO DISPERSIVE GEL USING SWOLLEN MICELLES TECHNIQUE AND ITS IN VITRO CHARACTERIZATION
Objective: The objective of the present work was to formulate and characterize nano dispersive gel (NDG) for topical delivery of water-insoluble antifungal agent ketoconazole in order to enhance its solubility, penetrati...
TRITERPENOID SAPONINS: A REVIEW ON BIOSYNTHESIS, APPLICATIONS AND MECHANISM OF THEIR ACTION
Saponins are the potential bioactive compounds secreted by plants, endophytic fungi and marine organisms. Saponins are the glycosides containing non sugar portion, aglycone (sapogenin) attached to sugar moiety by glycosi...
SYNTHESIS, CHARACTERIZATION, BIOLOGICAL EVALUATION AND DOCKING OF SOME NOVEL SUBSTITUTED 1, 3-THIAZINE DERIVATIVES
Objective: Chalcones and their heterocyclic analogs represent an important class of small molecules which have a wide range of pharmacological activities. Therefore, in this study, synthesis and anticonvulsant and antimi...
HEPATOPROTECTIVE ACTIVITY OF MAYTENUS EMARGINATA AGAINST PARACETAMOL INDUCED LIVER INJURY IN MALE WISTAR RATS
Objective: To study the hepatoprotective activity of ethanol extract of Maytenus emarginata against Paracetamol induced toxicity in male Wistar rats.Methods: The liver injury was induced by Paracetamol @ 500 mg/kg B.W. o...
ANTIOXIDANT ACTIVITY AND ANTIPROLIFERATIVE ACTION OF METHANOLIC EXTRACT OF LIQUORICE (GLYCYRRHIZA GLABRA) IN HEPG2 CELL LINE
Objective: To evaluate the in vitro antioxidant activity of liquorice (Glycyrrhiza glabra) against H2O2 induced oxidative stress in HepG2 cell line.Methods: Antioxidant activity of methanolic extracts of Glycyrrhiza glab...