Stability Indicating UPLC Method Development and Validation for Simultaneous Determination of Ertugliflozin and Sitagliptin
Journal Title: American Journal of PharmTech Research - Year 2019, Vol 9, Issue 0
Abstract
A selective, robust, economic and short method was developed for the simultaneous estimation of the Ertugliflozin (EGZ) and Sitagliptin (SGP) in Tablet dosage form. Chromatogram was run through X-bridge c18 50 x 2.1 mm, 1.7. Mobile phase containing Buffer: Acetonitrile taken in the ratio 50:50 was pumped through column at a flow rate of 1 ml/min. Buffer used in this method was 0.01N KH2PO4(3.0PH)buffer. Temperature was maintained at 30°C. Optimized wavelength selected was 240 nm Retention time of EGZ and SGP were found to be 0.883 min and 1.465 min. %RSD of the EGZ and SGP were and found to be 0.3 and 0.8 respectively. %Recovery was obtained as 99.83% and 100.09% for EGZ and SGP respectively. LOD, LOQ values obtained from regression equations of EGZ and SGP were 0.139, 0.421 and 0.18, 0.56 respectively. Regression equation of EGZ is y = 4300x + 253.6, and y = 6814x + 11844 of SGP. Retention times were decreased and that run time was decreased, so the method developed was simple and economical that can be adopted in regular Quality control test in Industries
Authors and Affiliations
Gupta V Rama Mohan
Keywords
Related Articles
- EP ID EP618967
- DOI 10.21276/ajptr.2019.09.01.07
- Views 187
- Downloads 0
Aromatic C-acylation of Phenols by using Acid and Acid Anhydride in Presence of Lewis Acid Catalyst
Ring acylations of aromatic substances are very difficult to achieve to a great extent. Nencki reaction has attempted on mono to poly hydroxy and substituted phenols have good results. Reactions are using solely acetic a...
Molecular Interaction and Excess Thermodynamic Parameters In Binary Liquid Mixture Through Ultrasonic Measurements at 313.15 K
Experimentally determined values of density ( ρ), viscosity (η) and ultrasonic velocity (u) have been measured for the binary liquid mixture toluene and 1,2 dichloroethane at 313.15K. From these data the thermodynamic...
Development of A Hospital Formulary In A Tertiary Care Hospital
One of the important fundamental human rights is access to health care. Various governments have shown international agreements about this right. Disease management and prevention is mainly through drugs. Complete inform...
A Review on Gastro Retentive Drug Delivery Systems with the Special Focus on Floating Drug Delivery
Gastro Retentive Drug Delivery System is one of the novel approach and all researchers in pharma industries are focused in the same. The main aim of writing this review on Gastro Retentive floating drug delivery systems...
Analytical Method Development and Validation for Enrofloxacin in Bulk and Formulation by RP-HPLC Method
Enrofloxacin (EFX) is a third generation Fluoroquinolone with a broad spectrum antibacterial activity. Enrofloxacin hydrochloride is 1-cyclopropyl-7-(4-ethylpiperazin-1-yl)-6-fluoro-4-oxo-1, 4-dihydroquinoline-3-carboxyl...