Standardized protocol for the in vitro culture of Artemisia annua L. – A medicinal plant at high altitudes of Nilgiris, the Western Ghats
Journal Title: Journal of Research in Biology - Year 2011, Vol 1, Issue 3
Abstract
A reliable protocol for callus induction and organogenesis, and successful plantlet survivability through hardening were developed for leaf explants of the medicinal plant species, Artemisia annua L. MS medium containing the auxin, NAA at 0.9mg/l is determined to be the optimum concentration for callus induction. Higher shooting (20.67/callus) was performed in the medium supplemented with BAP and GA3 at 0.5 and 1.0 mg/l respectively while subculturing the callus. Maximum number of roots (12.00 roots/shoot) was noted to be obtained in the medium containing IBA at 0.9mg/l. The in vitro regenerated plantlets were successfully acclimatized (86% survivability rate) in the hardening medium encomposing vermiculate, coir waste and forest litter in the ratio of 1:1:1 by volume
Authors and Affiliations
Ganesan CM, Paulsamy S
Keywords
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