<strong>Nodulation Competitiveness of <em>Ensifer meliloti</em> Alfalfa Nodule Isolates and Their Potential for Application as Inoculants</strong>
Journal Title: Polish Journal of Microbiology - Year 2014, Vol 63, Issue 4
Abstract
Alfalfa ([i]Medicago sativa[/i]) is widely cultivated legume, which enters into nitrogen-fixing symbiosis with [i]Ensifer[/i] ([i]Sinorhizobium[/i]) spp. In this study, autochthonous rhizobial population of [i]Ensifer [/i]sp[i].[/i] occupying alfalfa nodules grown in arable soil was used as the basis for selection of the potential inoculants. Alfalfa nodule isolates were identified as [i]Ensifer meliloti[/i] by partial 16S rDNA, [i]recA[/i], [i]atpD[/i] and [i]nodC[/i] nucleotide sequencing. The sampled isolates have shown different symbiotic performance and diversity in plasmids number and molecular weight. The isolates most efficient in the symbiotic nitrogen fixation were tagged with a constitutively expressed [i]gusA [/i]gene carried by a stable plasmid vector pJBA21Tc and used in competition experiments in soil under greenhouse conditions. Two [i]E. meliloti [/i]strains LU09 and LU12, which effectively competed with indigenous soil rhizobia, were selected. The metabolic profiles of these selected strains showed the differences in the use of carbon and energy sources. In addition, the LU09 strains revealed bacteriocin production and LU12 mineral phosphate solubilization, which are valuable traits for soil survival. These strains may be considered as potential biofertilizers for alfalfa cultivation.
Authors and Affiliations
Monika Marek-Kozaczuk, Jerzy Wielbo, Anna Pawlik
Keywords
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