Studies on Calli Mediated Multiple Shoot Regeneration from Rumex vesicarius L.
Journal Title: Biotechnology Journal International - Year 2014, Vol 4, Issue 5
Abstract
Rumex vesicarius L is a valuable potent medicinal herb, which belongs to family Polygonaceae. It possesses antimicrobial, anti- inflammatory, antidiarrhoeal and antioxidant properties. An attempt to study the In vitro callus induction and regeneration of plantlets from calli of leaf and nodal segments as explants has been achieved. Initially mature seeds were excised from plants grown in the departmental garden of KL University. The sterilized seed explants were inoculated aseptically to the solid basal SH media without any growth regulators for seed germination. Effective plantlets observed after 1 week of culture inoculation under maintained controlled conditions. From these In vitro plantlets, leaf and nodal segments were taken as explants for this study. These explants were inoculated on SH medium supplemented with different concentrations of BA (0.5-5.0mg/l) and 2, 4-D (0.5-3.0mg/l) for callus induction and multiple shoot regeneration. 90% of callus induction was observed on media containing BA 4.0mg/l and efficient multiple shoot induction (96.6%) was observed on media containing BA 1.0mg/l from leaf explants. 90% of callus induction and 85% of multiple shoot induction observed on media containing BA 2.0mg/l from nodal explants. Roots were induced from In vitro shoots on SH medium supplemented with 1mg/l IBA after 1 week. Leaf explants were more regenerative with 96.6% response compared to nodal explants 85%. Finally these In vitro regenerated plantlets were hardened, acclimatized and successfully transferred to the field. This protocol will be useful for mass multiplication of plantlets and maintenance of germplasm throughout the year.
Authors and Affiliations
Chakravarthy Rama, Lavanya Kakarla, Mahendran Botlagunta, M. S. R Krishna
Keywords
Related Articles
- EP ID EP237314
- DOI 10.9734/BBJ/2014/8375
- Views 119
- Downloads 0
Haploid Plant Regeneration from Unpollinated Ovules of Cucumis melo L. Var. Conomon cv. Mudicode
Major objective of the present work was to induce haploid plants in Cucumis melo var. conomon cv. Mudicode (Common name: Kani vellari). Ovules of Kani vellari plants were collected one day before anthesis and were cultur...
Genetics in Periodontics
Periodontal disease does not appear to be a single disease with variations in clinical symptoms but a group of diseases with overlapping symptomatology. The nature of periodontal diseases may be multifactorial. It will b...
Genetic and Phenotypic Identification of Vancomycin-Resistant Staphylococcus aureus Isolates from Retail Poultry Carcasses in Omu-Aran, North-Central Nigeria
Staphylococcus aureus is a well-known agent of zoonotic infections. Livestock-associated methicillin-resistant S. aureus (LA-MRSA) had been receiving public health attention for over a decade. Recently, the genomes of so...
Anti-tumor Effect of Calloselasma rhodostoma Venom on Human Breast Cancer Cell Line
Aim: Breast cancer is a major health issue for women worldwide. The potential anti-tumor effect of snake venom, has been studied and evidences showed reducing tumor size and inhibition of angiogenesis. Present study aims...
Biochemical Characterization, 16S rRNA Sequence Analysis and Multiple Sequence Alignment of Bacteria Isolated from Fermented and Unfermented Coconut
Coconut (Cocos nucifera L.) is a tropical and subtropical plant which has great versatility due to many benefits of its different parts. Appropriate methods such as biochemical, morphological, serological, physiological...