Studies on genotoxic, geno-protective and cyto-protective effects of Garcinia gummi-gutta extract against mitomycin C
Journal Title: Journal of Pharmaceutical and Biological Sciences - Year 2017, Vol 5, Issue 2
Abstract
Garcinia gummi-gutta, a tropical plant whose fruit rind is used as an herbal medicine for certain illness. However, reports on its genotoxicity and geno-protective effects are not available. Hydromethanolic rind extract of G. gummi-gutta fruit was prepared by maceration method and tested against mitomycin C, an anticancer drug. Experiments were conducted on Swiss albino mice to assess the possible dose-dependent effect of the extract (100, 200 and 300 mg/kg BW i.p). The selected doses of the extract were orally administered, and bone marrow micronucleus assay was performed. For the protective effect, the extract was orally gavaged 1 hr prior to mitomycin C (i.p. treatment -5mg/kg). Assay was performed for total antioxidant activity of the extract. Ascorbic acid was taken as the reference standard for both the assays. The extract did not induce the formation of micronucleated cells. However, the higher dose induced a significant decrease in the P/N ratio (p<0.05). Induction of micronucleated-cells and reduction in P/N ratio at a highly significant level (p<0.001) by mitomycin C confirmed its genotoxic and cytotoxic potency. The co-administration of the extract resulted in a dose-dependent protective effect against MMC-induced genotoxicity as indicated by reduction in the incidence of MN-cells. The extract also imparted the recovery of P/N ratio; 200mg/kg BW was found to be optimum dose. The extract exhibited the antioxidant activity (EC50 36.76±2.53) nearly 1/3rd of that of the ascorbic acid (EC50 13.65±1.26). Thus, the extract has substantial ameliorating effects against mitomycin C-induced geno- and cyto-toxicities, which can be attributed to its free-radical scavenging property.
Authors and Affiliations
Vishma B. L. , Prashantha Naik, Sujayraj R. S
Keywords
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