STUDIES ON MOLECULAR CHARACTERIZATION OF RAGI BLAST PATHOGENS FOR VARIABILITY AMONG PYRICULARIA GRISEA (CKE) SACC. ISOLATED FROM VARIOUS REGIONS OF TAMIL NADU BY RAPD MARKERS ANALYSIS
Journal Title: International Journal of Agriculture Sciences - Year 2016, Vol 8, Issue 14
Abstract
Pyricularia grisea (Cke) Sacc incites blast disease in finger millet and rice. An r-DNA region was amplified from the 12 isolates of P. grisea (B1, B2, B3, B4, B5, B6, B7, B8, B9, B10, B11 and B12) isolated from finger millet and rice, using the universal primers to form a phylogenetic tree revealed that the isolates fall in 3 different clusters. Where in, ITS1-5.8S – ITS5 sequences and Randomly Amplified Polymorphic DNA profiling of P.grisea isolates used in the study clustered into two main groups, in which the isolates B1, B2, B3, B4, B5, B6, B7, B8, B9, B10 and B11 formed one group with approximately 12 per cent similarity coefficient between them and B12 was grouped in separate cluster. Molecular characterization of ragi blast pathogens in the study by RAPD markers analysis segregated the isolates into different clusters confirmed that the rice and finger millet-infecting P. grisea populations of India are clearly distinct.
Authors and Affiliations
ARUNKUMAR NAGARATHINAM
Keywords
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