Study Cultural, Morphological and Pathogenic Variation among Different Isolates of Fusarium oxysporum f. sp. lentis
Journal Title: International Journal of Current Microbiology and Applied Sciences (IJCMAS) - Year 2018, Vol 7, Issue 9
Abstract
Lentil (Lens culinaris Medik.), one of the major pulses cultivated and consumed in India, is also known as Masur. It has a high nutritive value and major source of dietary proteins (25%) after soybeans in human and animal diet. Fusarium wilt of lentil caused by Fusarium oxysporum f.sp. lentis is the most serious disease of lentil. Ten isolates of F. oxysporum f.sp. lentis were studied for its cultural, morphological and pathogenic variability. Pigmentation is varied among the isolates. Out of 10 isolates, 4 isolates FOL-R3, FOL-V8, FOL-M6 and FOL-R9 were Purple, were dull yellowish, isolates FOL- B7, and FOL-S10, four isolates like FOL-A2, FOL-L4, FOL-S5 and FOL-S10 were white FOL-F1was light puff in colour. The size of microconidia varied between7.30×3.43 (FOL-L4) to 13.25 x 2.68μm (FOL-S10) and macroconidia varied from 24.11×4.67 (FOL-A2) to 40.05 x 4.71μm (FOL-V8). The number of septa in macro-conidia was mostly 3-6 and micro-conidia are mostly no septum and some are 0-1. Chlamydospores are terminal and intercalary. Pathogenic variability revealed that most of the isolates were highly pathogenic.
Authors and Affiliations
Khushboo Dubey, Sushil Kumar Singh
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