Study of analyte stability time of serum electrolytes in a tertiary care public hospital
Journal Title: Medpulse International Journal of Biochemistry - Year 2019, Vol 10, Issue 3
Abstract
Background: The collected and shipped blood samples are exposed to various extra-analytical factors prior to analysis. At times there is a delay in sample analysis after centrifugation due to a large sample load, breakdown of the analyzer and the nonavailability of a backup system. With this back ground we took up this study to see the effect of time on the assayed values of serum electrolytes.Material and Methods: We analyzed 152 samples for serum electrolytes at different time intervals between centrifugation and sample analysis on automated analyzer. The samples were received from different wards within one hour after sample collection. The samples were immediately analyzed after centrifugation and values recorded. The samples were kept in the lab uncovered as per the usual practice, with controlled environmental temperature conditions (24 ± 2˚C) and were estimated twice after a gap of 2 hours each. i.e. at 3 hours and 5 hours post sample collection. All the samples were analyzed on the same instrument using Beckman AU680 analyzer on ISE mode.Results: Results compared using repeated measure ANOVA which showed statistical difference P < 0.05 between 1st and 2nd, and 2nd and 3rd measurement. There was statistically significant rise in values of Na and K over a period of time.Conclusion: Evaporation of sample could be the major cause leading to sample concentration resulting in high values. Simple measures like early analysis of samples or covering the samples properly will prevent such erroneous results.
Authors and Affiliations
Dipti Tiwari, Pramod Ingale, Shubhangi Wankhade, Minal Pore
Keywords
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- EP ID EP613249
- DOI 10.26611/10021031
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