Study of biofilm producing capacity of pathogens isolated from different catheters
Journal Title: Medpulse International Journal of Microbiology - Year 2017, Vol 4, Issue 1
Abstract
Background: Persistent infection due to biofilm formation is certainly a new and additional burden to clinicians who treat infections. The purpose of this study is to determine the biofilm producing capacity of pathogens isolated from different catheters. Material and Methods: A total of 109 catheter samples from 100 adult patients of both sexes admitted to the Intensive Care Units (ICUs) who have been catheterized for more than 48 hrs showing clinical signs of sepsis. Maki’s semi-quantitative method was used to culture thecatheters and biofilm detection was done by culture plate assay. Results: Out of the 109 catheters studied and cultured, a total of 26 isolates were obtained. Coagulase negative staphylococci and Escherichia coli formed the most commonly isolated pathogens at 5 (19.2%) each. Among the 26 isolates cultured, 22 of the organisms produced biofilms as evidenced by the tissue culture plate assay. Conclusion: Biofilm formationpose a public health problem for the persons who require indwelling medical devices. Its detection should be mandatory in a laboratory set up. Tisue culture plate method is an accurate and reproducible screening method for biofilm production.
Authors and Affiliations
Harsha Vardhana S, P R Sreenivasa Babu, M R Sandhya Belawadi
A trend of antibiotic resistance pattern of klebsiella pneumoniae isolated from various clinical samples in patients attending tertiary care hospital Saurashtra region, Gujarat, India
Background: Klebsiella pneumoniae has been associated with different types of infections and one of the most important aspects of Klebsiella pneumoniae is emergence of multi-drug resistant strains. Moreover, extensive us...
Chlamydial infection association with syphilis in patients attending STD clinic in a tertiary care hospital
Background: Co-infection of Chlamydia with syphilis highlights the importance of early laboratory diagnosis and specific treatment. It is worthwhile to screen patients for this infection and to initiate prompt treatment...
Biofilm production among coagulase negative staphylococci and its correlation with antimicrobial susceptibility pattern
Background: Coagulase negative staphylococci (CoNS), which are the normal skin flora, have emerged as predominant pathogens in hospital-acquired infections. An important step in the development of catheter or implant-ass...
Invasive candidiasis outbreak in a neonatal intensive care unit (NICU), a descriptive study from tertiary care teaching hospital of western part of Maharashtra
Background: Candidemia outbreaks are frequent event take place in NICU accounting about 9-13%. Especially non-albicans Candida species emerged as an important cause of various outbreaks in NICU. There are various predisp...
Comparison of rapid test and conventional staining methods for the diagnosis of malaria in a tertiary care hospital
Rapid diagnosis is extremely important for effective treatment and reducing the mortality and morbidity of malaria. Therefore, study was undertaken to compare the efficacy of conventional staining methods (Leishman stain...