Study of expression patterns of cytokeratin 8 and 18 in oral cancers
Journal Title: Pathology Update: Tropical Journal of Pathology and Microbiology - Year 2016, Vol 2, Issue 3
Abstract
Introduction: Squamous cell carcinoma of oral cavity is a morphological heterogenous disease. Cytokeratin (CK) comprises a diverse group of intermediate filament protein that are expressed as pairs in both keratinized and non keratinized epithelial tissue. Very little is known of CK alteration in oral intraepithelial neoplasia III (OIN III) and invasive squamous cell carcinoma (SCC).The aim of the study is to evaluate the expression pattern of CK8/18 in oral carcinoma and to establish any correlation between the expressions of CK8/18 with tumor differentiation. Material and Methods: A total of 104 cases of Paraffin embedded formalin fixed sections from oral squamous cell carcinomas (N=60) including benign and inflammatory lesions (control;N=40) and oral intraepithethelial lesions(N=4) were stained with Haematoxylin and eosin stain and and then studied immunohistochemically using CK8 and CK18 immunomarkers. Result: Out of 60 cases of invasive squamous carcinomas, cytokeratin 8 showed positivity in 34 cases (56.67%) while expression of cytokeratin 18 was observed in 25 cases(41.67%) but its expression were absent in control and oral intraepithelial cases. Expression of CK 8 was seen in majority of poorly differentiated 4/5(80%) followed by moderately differentiated 12/17(70.59%), with its expression were significantly higher (56.67%) in invasive oral carcinomas compared with controls statistically .Similarly CK18 expression was seen in majority of poorly 4/5 (80%) followed by moderately differentiated squamous cell carcinomas 7/17(41.18%). Conclusion: Aberrant expression of CK8/18 could be correlated with differentiation and recurrence and shows a great promise to be used as prognostic marker for human oral cancer.
Authors and Affiliations
Shaila Kumari Mitra, R. K. Misra, Rajesh Rai, Priyanka Rai, Murari Lal Arya
Keywords
Related Articles
- EP ID EP228469
- DOI 10.17511
- Views 191
- Downloads 0
BRCA1 Positivity in Triple Negative Breast Cancers: A Prospective Study in Tertiary Care Cancer Hospital
Objectives: In the present study we investigated the BRCA1, Estrogen receptors status (ER), progesterone receptor status (PR) and human epidermal growth factor receptor (Her2neu) expression in selected patients with high...
Diagnostic accuracy of Bronchoalveolar lavage fluid in diagnosis of lung cancers in a tertiary care hospital in coastal region of Karnataka
Introduction: Bronchoalveolar lavage (BAL) fluid analysis helps in early detection, rapid diagnosis and treatment of lung cancer as the therapy is based on subtyping. BAL has an important role in diagnosis of peripherall...
A histomorphological study of trichilemmal cysts: a rural hospital experience
Introduction: Among Cystic lesions of skin the most common clinical diagnosis is sebaceous cyst. Cystic lesions of the skin are most commonly encountered in surgical practice. Among these cyst lesions Trichilemmal cysts...
Comparative study using routine stains and Immunohistochemistry staining techniques for detection of Helicobacter pylori
Introduction: The aim of this study was to evaluate the role of endoscopic biopsies for detection of Helicobacter Pylori using routine staining techniques which included hematoxyline and eosin, Giemsa and Immunohistochem...
Study of blood donor profile in a blood bank attached to a medical college hospital - a retrospective study
Background: Blood Transfusion Services (BTS) are the vital part of modern health care system without which efficient medical care is impossible. To make blood donation safe and increase the confidence of the masses in vo...