Study of Nuclear Anomalies and Cytological Features in Buccal Mucosa of Tobacco Users
Journal Title: International Journal of Contemporary Medical Research - Year 2017, Vol 4, Issue 11
Abstract
Introduction: Tobacco, a chemical carcinogen, has genotoxic effects on buccal mucosal cells. The present study is performed to evaluate all cytological features in exfoliated buccal mucosal cells of tobacco users and to compare them in different form of tobacco users i.e. smokers and smokeless “chewers”. Also to evaluate whether Micronuclei frequency can be used as a biomarker and screening tool to assess genotoxicity in tobacco users. Material and methods: Oral buccal mucosal exfoliated cells from total 80 tobacco users-40 smokers and 40 smokeless tobacco chewers, and 20 contols with healthy mucosa were taken. Micronuclei frequency and other nuclear anomalies were compared using Papanicolaou stain. The results are analyzed statistically using t-test and Fisher’s Exact test. Results: Smears of individual with tobacco habits showed significant increase in Mean Micronuclei (MN) per 100 screened cells (p=0.01). Cytological features like orangophilia, keratinisation, degenerative changes and all nuclear anomalies are increased in tobacco users but only nuclear fragmentation show statistically higher incidence. No statistical significant difference was found for Mean MN and other nuclear anomalies between tobacco smokers and smokeless tobacco “chewers”. Conclusion: Nuclear anomalies and Mean MN frequency are increased in any form of tobacco users, thus MN frequency can be used as a biomarker and a screening tool for genotoxicity in any form of tobacco users.
Authors and Affiliations
Seema Baxi, Mayuri Gohil
Keywords
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