Study of stability of experimental living vaccine against animal anthrax with strаin UA–07 “Antravak” (18 months after production)
Journal Title: Біологія тварин - Year 2018, Vol 20, Issue 1
Abstract
For the prevention of anthrax, various vaccines are developed. The article presents the results of investigations on determination of the experimental live vaccine stability against anthrax from animals of the genus Bacillus anthracis UA–07 “Antravak”. Experimental vaccines of series 1 and 2 were studied according to the following indices: appearance, presence of foreign impurities, mass fraction of glycerin, pH, amount of live spores, mass fraction of spores, contamination, growth typicality, homogeneity, morphology, mobility, capsule formation, harmlessness, residual virulence, immunogenic activity, storage temperature. The stability of the studied parameters of the vaccine series 1 and 2 during the research period (6, 12 and 18 months after tpriduction) was established. In the study the mass fraction of glycerin was revealed 29–31%, pH was within 7,5–7,7, the amount of live spores in 1 cm3 was found within 13,48–14,23 million; the vaccine did not cause swelling, skin necrosis, animal death, it was immunogenic for 90 % experimental animals. In the study of the parameters of the vaccine series 2, the absence of foreign impurities, bacterial and fungal microflora was revealed. According to the results of the research, it was found that the experimental vaccine against anthrax from Bacillus anthracis UA–07 “Antravak” strain of series 1 and 2, regardless of storage temperature, was almost constant in the study.
Authors and Affiliations
I. A. Rublenko, V. G. Skripnik
Keywords
Related Articles
- EP ID EP356832
- DOI 10.15407/animbiol20.01.116
- Views 76
- Downloads 0
Histomorphology of organs and activity of some enzymes in mice after immunization with polymer based on acrylic acid or aluminum hydroxide as adjuvants
Vaccination is the best biomedical approach in avoiding diseases. Proteins and peptides purified from microorganisms or synthesized chemically are weakly antigenic and need adjuvant to provide strong immune responses. Ma...
Transfusion of canine cryopreserved red blood cells in treatment of babesiosis
The results of the evaluation of the clinical and biochemical efficiency of dogs cryopreserved red blood cells transfusion with acute form of babesiosis are presented. Cryopreserved erythrocyte mass was obtained by rapid...
The influence of surfaces modified by different polymeric substances on the growth of mouse embryonic fibroblasts cell line NIH3T3
Cultivating cells on synthetic surfaces has ensured the most approximated to in vivo conditions, cell growth, migration, differentiation, synthesis of the extracellular matrix components. Cell proliferation and viability...
Determination of antibacterial properties of triazines in regards to Salmonella typhimurium
The results of the study of antibacterial activity and determining minimum inhibitory concentrations triazines classes by microdilution method against a Salmonella typhimurium are described in the article. The antimicrob...
Comparative analysis of genetic structure of a micro-population of gray Ukrainian breed cattle by DNA markers
The use of advances in genetics in cattle breeding from identification to parentage determination significantly improves its efficiency. The genotyping of animals by means of microsatellite loci is mandatory for recognit...