Study of superoxide- and NO-dependent protective mechanisms of N-acetylcysteine and losartan in ratʼs aorta and liver under streptozoticin-induced type 1 diabetes mellitus
Journal Title: ScienceRise: Pharmaceutical Science - Year 2017, Vol 0, Issue 6
Abstract
<p><strong>Aim.</strong> The aim of the research was to investigate superoxide- (SR) and NO-dependent mechanisms of action of N-acetylcysteine (NAC) and losartan (LOS) in aorta and liver of rats with type 1 diabetes mellitus (DMI).</p><p><strong>Methods.</strong> Diabetic rats were treated with NAC (1.5g/kg), LOS (20mg/kg) or combination (NAC+LOS) for 4 weeks.</p><p><strong>Results.</strong> The rate of SR generation by mitochondria of aorta in untreated diabetic animals was significantly higher than in control, while the level of NO was decreased. Production of SR and NO in the liver of diabetic rats were dramatically increased. The marker of oxidatively damaged DNA, 8-oxoG, was raised in urine of diabetic rats. All of pharmacological schemes showed significant decrease the rate of SR generation by mitochondria of aorta and liver; NO level was lowered in the liver tissue compared to DMI. Only NAC significantly restored NO level in aorta of diabetic rats. Intervention with NAC / LOS or NAC+LOS increased the level of 8-oxoG in compare to DM1.</p><p><strong>Conclusion.</strong> In conclusion, treatment with NAC and LOS or combination is associated with protection of aorta and liver cells in diabetic animals against toxic action of SR, preventing mitochondrial dysfunction and further DNA damage, which point out the cardioprotective effects and overall metabolic improvement</p>
Authors and Affiliations
Inna Sytnyk, Anatoliy Burlaka, Anastasia Vovk, Mykola Khaitovych
Keywords
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- EP ID EP647878
- DOI 10.15587/2519-4852.2017.119490
- Views 146
- Downloads 0
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