Study on fungal contaminants in callus formation from foliar explants of Coffea sp.
Journal Title: Biotecnología Vegetal - Year 2003, Vol 3, Issue 2
Abstract
The coffee is a crop of economic importance for several developing countries and the application of the biotechnology offers advantages in its propagation. Nevertheless, the microbial contamination continues being one of the problems that affects the in vitro cultuture. This paper had as objectives to identify fungal contaminants during the phase of callogenesis of Coffea arabica and Coffea canephora, as well as to evaluate the effect of the RIZOBAC in the control of the same ones. Samplings to obtained callus of leaf explants were carried out, discarded by microbial contamination and they were isolated and identified fungal contaminants, considering cultural and morphological characteristic. The antagonistic effect of RIZOBAC against the strains of fungal contaminants was determined, being inoculated 100 μl of RIZOBAC in badges with medium Potato Dextrose Agar on those that suspensions of spores of the isolated strains were placed. The incubation was carried out at 28 °C for 7 days. The diameter of the colonies of the fungi was measured and the inhibition percentage was determined. The percentages of fungous contamination in the explantes oscillated between 51.2 and 73.7 %. The filamentous fungi of more appearance frequency were Fusarium and Cladosporium. The antagonistic effect of the RIZOBAC was demonstrated, obtaining between 71.5 and 43.3 % inhibition of the fungi growth, respectively, constituting a viable alternative the use of this product in order to diminish the indexes of fungous contamination in the in vitro culture.
Authors and Affiliations
María Esther González, Luis Manuel Barrios
Keywords
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