Study on the growth inhibitory effects of cartilage polysaccharide<br /> on L1210 cells<br />
Journal Title: Science Paper Online - Year 2009, Vol 4, Issue 9
Abstract
To investigate the growth inhibitory effect of cartilage polysaccharide on L1210 leukemia mice，and discuss its anti-tumor mechanism. DBA/2 mouse model was established using mouse L1210 cells. Mice were divided into 5 groups，and were treated by i.P. injection of cartilage polysaccharide. Body weight was measured every day and survival time was recorded. The increase of lifespan was calculated. Giemsa staining were performed using ascites got from the mouse in treated group at 0 h, 24 h, 48 h and 72 h to observe the changes of morphology. The apoptotic cells were detected by TUNEL assay and the expression of Bax and Bcl-2 proteins were studied by immunofluorescent technology to explore the anti-tumor mechanism of cartilage polysaccharide. The survival rate of L1210-bearing mice increased significantly by treatment of cartilage polysaccharide．Cell shrinkage, nuclear condensation and apoptotic body could be detected by cell morphological observation．The apoptotic rate significantly increased on 72 h vs. 0 h(P＜0.01)．The expression of Bax protein was up-regulated after treatment of cartilage polysaccharide as well as the expression of Bcl-2 expression was low-regulated. Apoptosis of L1210 cells can be induced by cartilage polysaccharide through the effect on the expression of Bax an d Bcl-2 proteins, and the great inhibition on tumor cell growth．
Authors and Affiliations
Ning Yang, Anjun Liu
Keywords
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