Study on the hepatitis B virus infection in blood donors reactive to jointed NAT but non-reactive to primary discriminatory test
Journal Title: Chinese Journal of Blood Transfusion - Year 2022, Vol 35, Issue 11
Abstract
Objective To analyze the status of HBV infection in blood donors reactive to jointed NAT but non-reactive to primary discriminatory tests (NRR), so as to provide suggestions and data support for subsequent studies on NRR samples. Methods HCV RNA and HIV RNA repeat differential detection, HBV DNA viral load detection and HBV pgRNA copy volume detection were performed in the plasma of 60 blood donors with negative ELISA results in routine blood screening and NRR in NAT. HBsAg, HBsAb, HBcAb, HBeAg and HBeAb serological tests were performed on the NRR samples with positivity in HBV DNA viral load and HBV pgRNA virus copy detection, so as to analyze the serological infection status and occult hepatitis B (OBI) infection. Results The HCV RNA and HIV RNA repeat discrimination results of 60 NRR samples were negative. The quantitative detection results of HBV DNA in 60 NRR samples were positive in 9 cases (15%), and the HBV DNA concentration was less than 10IU/mL. Nine cases (15%) were positive for HBV pgRNA quantitative detection, and the virus copy volume ±SD was (289±58.25) copies/mL. Two NRR samples (3.33%) were HBV DNA positive and HBV pgRNA positive. Among the 9 HBV-DNA positive samples, the highest positive rate of HBcAb was 66.67%, and 7 (77.78%) of them were confirmed to be seropositive for OBI. Among the 9 HBV pgRNA positive samples, the copy amount of pgRNA in HBcAb positive samples was slightly higher than that in negative samples, while the copy amount of pgRNA in HBsAb and HBeAb positive samples was lower than that in corresponding negative samples. In recent 6 years, the proportion of NRR samples in the single NAT system of the center fluctuated from 0.09% to 0.13%. Conclusion HBV DNA and HBV pgRNA exist in NRR samples. HBV DNA and/or HBV pgRNA positive samples can be detected in the relevant serological infectious markers. NRR samples have a certain potential risk of OBI infection. HBV DNA detection plus HBV pgRNA can better confirm the status of virus infection in NRR and improve the safety of blood transfusion.
Authors and Affiliations
Fengyuan LI, Tong PAN, Xia WANG, Fuhua ZHANG, Huafei GONG
Keywords
Related Articles
- EP ID EP738401
- DOI 10.13303/j.cjbt.issn.1004-549x.2022.11.006
- Views 85
- Downloads 0
Bibliometrics and visual analysis of platelet rich plasma based on strategic coordinate diagram and co-word analysis
Objective To explore the research status, hot spots and trends of platelet rich plasma (PRP). Methods With "platelet rich plasma (PRP)" and its Chinese equivalent as the subject words, the PRP related articles during Jan...
Electrolyte changes induced by massive transfusion in patients undergoing cardiac surgery
Objective To investigate the effect of electrolyte concentration on transfusion related complications in patients undergoing cardiac surgery after short-term massive transfusion (MT). Methods Seventy-six cases of adult c...
Three platelet antibody detection methods: a comparative study
Objective To compare three kinds of platelet antibody detection methods used to identify alloantibodies in patients with platelet transfusion refractory(PTR). Methods The 83 samples from PTR patients were analyzed base o...
The risk of Alzheimer′s disease transmitted by blood transfusion based on APP/PS1 double transgenic mice
Objective To explore the risk of Alzheimer′s disease (AD) transmitted by blood transfusion. Methods There were 10 APP/PS1 mice of 3, 6 and 9 months old, half female and half male, and the cognitive and behavioral abiliti...
A study of centralized blood testing in Sichuan province based on cost analysis
Objective With the development of blood transfusion technology, the blood testing model is relatively decentralized, inefficient and costly in blood stations in China. Blood centralized detection not only effectively imp...