Studying optimum conditions for biofilm production from Leuconostoc mesenteroides
Journal Title: Journal of Research in Ecology - Year 2018, Vol 6, Issue 2
Abstract
A series of experiments were conducted to identify the optimal conditions for biofilm production including physio-chemical substances such as : sucrose, tween 80, glycerin, sorbitol and other physical-chemical factors (temperature, pH). Different degrees of temperature, were used (25, 30, 35 and 40°C), also different pH were used (5.5, 6.0, 6.5, 7.0) in different incubation periods, (24, 48, 72 and 96 h) and at different sucrose concentrations (5, 10 and 15%). Bacterial activity in biofilm production is estimated under each factor by using Microtiter Plate method (MTP). It was found that the highest activity was achieved under this factors: fermentation temperature of 30°C, initial pH of 6.0, incubation period of 72 h, with medium contains sucrose (10%) and 1% of tween 80
Authors and Affiliations
Abdullah Ismaeel Awad, Asmaa Sabah Ahmaed
Keywords
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