SUPER CRITICAL FLUID CHROMATOGRAPHY: AN OVERVIEW
Journal Title: Int J of Pharm Res & Analy - Year 2014, Vol 4, Issue 7
Abstract
Supercritical Fluid Chromatography (SFC) is a form of normal phase chromatography, first used in 1962.SFC typically utilizes carbon dioxide as the mobile phase; therefore the entire chromatographic flow path must be pressurized. Because the supercritical phase represents a state in which liquid and gas properties converge, supercritical fluid chromatography is sometimes called "convergence chromatography." Supercritical fluid chromatography is one of the most important column chromatography methods after gas chromatography (GC) and high-performance liquid chromatography (HPLC). Supercritical fluids combine useful properties of gas and liquid phases. The characteristic properties of a supercritical fluid are density, diffusivity and viscosity.SFC, the sample is carried through a separating column by a supercritical fluid where the mixture is divided into unique bands based on the amount of interaction between the individual analytes and the stationary phase in the column. As these bands leave the column, their identities and quantities are determined by a detector SFC is a hybrid of gas and liquid chromatography because when the mobile phase is below its critical temperature and above its critical pressure, it acts as a liquid, so the technique is liquid chromatography (LC) and when the mobile phase is above its critical temperature and below its critical pressure, The instrumentation that is required for supercritical fluid chromatography is versatile because of its multi-detector compatibility.SFC has been applied to wide variety of materials including natural products, drugs, foods, pesticides, herbicides, surfactants, polymers and polymer additives, fossils fuels, petroleum, explosives and propellants.
Authors and Affiliations
Ranjeet Kumar V*, , SaiMalakondaiah D, , Ajitha A, , UmaMaheshwaraRao V
Keywords
Related Articles
- EP ID EP101172
- DOI -
- Views 144
- Downloads 0
CAPILLARY ELECTROPHORESIS INDUCTIVELY COUPLED MASS SPECTROMETRY
Capillary electrophoresis (CE) is a separation technique in which the analytes are separated on the basis of differences in their charge-to-size ratios. This makes CE especially suitable for the analysis of molecules wi...
SIMULTANEOUS QUANTIFICATION OF EPIGALLOCATECHIN-3-GALLATE AND ATORVASTATIN BY USING HPLC-UV METHOD AND ITS APPLICATION TO PHARMACOKINETIC STUDIES IN RATS
A sensitive, precise and simple LC method for the simultaneous quantification of epigallocatechin-3-gallate and atorvastatin in rat plasma has been developed and validated. The chromatographic separation was achieved on...
DEVELOPMENT AND VALIDATION OF HPLC METHOD FOR DETERMINATION OF ROXITHROMYCIN FROM HUMAN PLASMA
A stable, simple, rapid, precise, accurate HPLC method for analysis of Roxithromycin was developed and validated as per ICH guidelines without need of any internal standard. Separation was carried out using X’terra...
SEASONAL WATER QUALITY ASSESSMENT OF SURFACE WATER IN DURG REGION
An investigation was undertaken to determine the Quality of surface water of Shivnath River & ponds in durg and rajnandgaon region”. The study assessed the evolution of water quality in Surface of Durg and Rajn...
HPLC METHOD DEVELOPMENT AND VALIDATION: AN OVERVIEW
High performance liquid chromatography (HPLC) is an analytical technique which is proficient to separate, detect and quantify various drugs and its related degradants. An appropriate mobile phase, column, column temperat...