Suppressive Effects of Intrathecal Application of Diazepam on Visceral Pain and Hyperalgesia Induced by Intracolonic Instillation of Formalin
Journal Title: International Journal of Biomedical Science (IJBS) - Year 2006, Vol 2, Issue 1
Abstract
Using an animal model of colonic inflammation, the effects of intrathecal (i.t.) diazepam (0.02mg/kg, 0.08mg/kg and 0.15mg/kg) on visceral pain-related behaviors and hyperalgesia associated with colonic inflammation were investigated. In this visceral pain model, acute visceral pain response was induced by intracolonic (i.c.) injection of 0.5 ml of dilute formalin (2%, 5% and 10%) in rats, and the peak pain behavioral response and hyperalgesia were evoked by i.c. 5% formalin. I.t. diazepam (0.02mg/kg, 0.08mg/kg and 0.15mg/kg) followed 10 min later by i.c. injection of 5% formalin, attenuated the visceral pain behaviors induced by 5% formalin in a dose dependent manner. Of the three doses tested, the duration of the suppressive effect of 0.15mg/kg diazepam on visceral pain was the longest, which is 60 min compared with 45 min at other two doses. Moreover, i.t. pretreatment with 0.08mg/kg diazepam attenuated the hyperalgesia induced by i.c. injection of 5% formalin. The findings in our studies shown that i.t. diazepam had a suppressive effect on visceral pain associated with noxious stimulation of colon, and provided evidence that diazepam may be used as an analgesic drug in the future. How to cite this article: Int. J. Biomed. Sci. 2006; 2(1):64-72 For full text article or PDF file, visit: www.ijbs.org
Authors and Affiliations
Jinghui Huang, Libing Liu, Yumei Zhou, Jun Yu, Jiao Deng
A Role of CREB in BRCA1 Constitutive Promoter Activity and Aromatase Basal Expression
Aromatase is the rate-limiting enzyme in estrogen biosynthesis and a key target in breast cancer treatment. Its ovary-specific promoter, pII, is induced in response to protein kinase A (PKA) activation. It has been propo...
EMRSA-15 Bacteremia is not Associated with a Worse Outcome Compared with Bacteremia Caused by Multidrug-Resistant MRSA
EMRS A-15 (ST 22-MRS A-IV) is rapidly replacing the endemic ST 239 health care-associated methicillin- resistant Staphylococcus aureus clone in Singapore. A one-year single-centre cohort study of inpatients with MRS A ba...
TP53 codon 72 Gene Polymorphism Paradox in Associated with Various Carcinoma Incidences, Invasiveness and Chemotherapy Responses
TP53 is the most common mutated gene in human cancers. Approximately half of all human malignancies exhibit TP53 mutations. The TP53 codon 72 polymorphism is a single-nucleotide polymorphism (SNP) in exon 4, resulting in...
Changes in Mice Brain Spontaneous Electrical Activity during Cortical Spreading Depression due to Mobile Phone Radiation
The objective of the present study was to investigate changes in spontaneous EEG activity during cortical spreading depression (CSD) in mice brain. The cortical region of anaesthetized mice were exposed to the electroma...
HIV/AIDS Knowledge, Attitudes, and Behaviors of Construction Workers in China
The objective of the study was to describe HIV/AIDS knowledge, attitudes, risk behaviors, and sources of information among construction workers in China. A cross-sectional survey of 458 construction workers was conducted...