SURFACE STERILIZATION PROTOCOL FOR Curcuma angustifolia ROXB. MICROPROPAGATION
Journal Title: Journal of Experimental Biology and Agricultural Sciences - Year 2018, Vol 6, Issue 5
Abstract
For the micropropagation of Curcuma angustifolia usually rhizome buds are used as explants which are definitely associated with various types of soil-born microorganisms, therefore only one type of sterilant cannot be used for the sterilization of these rhizome buds. Therefore present investigation was carried out to develop the most effective surface sterilization protocol for C. angustifolia. In the present study, explants were treated with three sterilizing agents viz., fungicide (Dhanustin) at 1 % (m/v), 70 % (v/v) ethanol and mercuric chloride (HgCl2) at a concentration of 0.1 % (m/v). Among the different used sterilants with varying treatment time, 1 % (m/v) fungicide with 4 drops of Tween 20 for 30 minutes followed by 70 % alcohol for 1 minute and lastly 0.1 % (m/v) HgCl2 for 10 minutes was found most effective surface sterilizing combination for C. angustifolia micorpopagation.
Authors and Affiliations
Ningthoujam Sandhyarani Devi, RK Imotomba, Rocky Thokchom
Keywords
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- EP ID EP593090
- DOI 10.18006/2018.6(5).890.894
- Views 59
- Downloads 0
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