Synthesis, characterization and bioevaluation of technetium-99m labeled N-(2-Hydroxybenzyl)-2-amino-2-deoxy-D-glucose as a tumor imaging agent
Journal Title: Pakistan Journal of Pharmaceutical Sciences - Year 2013, Vol 26, Issue 2
Abstract
N-(2-Hydroxybenzyl)-2-amino-2-deoxy-D-glucose (NHADG) was synthesized by conjugation of salicylaldehyde to glucosamine. The obtained compound was well characterized via different analytical techniques. Labeling of the synthesized compound with technetium-99m (99mTc) in pertechnetate form (99mTcO4-) was carried out via chelation reaction in the presence of stannous chloride dihydrate. Maximum radiochemical yield of 99mTc-NHADG complex (99%) was obtained by using 1 mg NHADG, 200 µg SnCl2.2H2O, at pH 9.5 and reaction time of 15 min. The radiochemical purity of the 99mTc-NHADG complex was measured by Instant Thin Layer Chromatography (ITLC) and Paper Chromatography (PC), without any notable decomposition at room temperature over a period of 4h. The biological evaluation results show that the 99mTc labeled NHADG conjugate is able to specifically target mammary carcinoma in mice models, thus highlighting its potential as an effective 99mTc labeled glucose-derived agent for tumor imaging.
Authors and Affiliations
Irfan Khan, Muhammad Javed, Qaisar Nadeem, Zaid Mahmood, Ume-Kalsoom Dar, Muhammad Ali, Syed Hyder, Sohail Murad
Keywords
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