SYNTHESIS OF N-4-METHOXYBENZOYL-N'-PHENYLUREA COMPOUND AND ACTIVITY TEST OF ANTICANCER AGAINST HeLa CELL LINE
Journal Title: World Journal of Pharmaceutical Research - Year 2018, Vol 7, Issue 4
Abstract
Research in the search for cancer drug compounds continues to be developed, considering that specific anticancer compound has not been obtained. Some of the urea derivative compounds are also being developed in the search for an anti-cancer compound which is potent with minimal side effects. Related to the explanation above, a urea derivative, which is N-phenylurea, would like to be developed. It would be reacted with 4-methoxybenzoyl chloride, thus N-4- methoxybenzoyl-N'-phenylurea compound would be obtained. The synthesis of N-4-methoxybenzoyl-N'-phenylurea was carried out by Schotten-Baumman method that had been modified, and then a test of purity was done by thin layer chromatography using 3 different kinds of eluent. The next phase was structure characterization which was carried out by using UV and IR spectrophotometry, then 1H-NMR spectrometry also MS, so that the structure from N-4- methoxybenzoyl-N'-phenylurea would be obtained. Anticancer activation test was conducted on HeLa cell line by using MTT assay, and the value of IC50 would be obtained. The compound that has been successfully synthesized is N-4-methoxybenzoyl-N'-phenylurea, with a yield of 51,23%. The purity test of N-4-methoxybenzoyl-N'-phenylurea was done by thin layer chromatography with 3 different types of eluent (hexane: ethyl acetate: methanol = 2: 3: 1; Hexane: acetone = 4: 2; Hexane: ethyl acetate = 4: 2) one single spot was obtained, which its value of Rf is different than the original compound, N-phenylurea. While the melting point of the compound is 158oC, thus it was seen that the compound has been formed and was different from the original compound, N-phenylurea. Anticancer activation test has been performed with MTT assay method by using HeLa cell line, the result obtained of IC50 is 6.50 mM more active than hydroxyurea is 170.57 mM as a standard. The conclusion is that N-4-methoxybenzoyl-N'phenylurea, have been successfully synthesized and has anticancer activity.
Authors and Affiliations
Bambang Tri Purwanto
Keywords
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