Abstract

A series of fourteen 3, 4, 5-trihydroxy benzoic acid were synthesized by schematic route shown in scheme 1 and characterized by spectroscopic analysis. All the newly synthesized derivatives were evaluated for their antimicrobial activity against Gram-positive bacteria: Staphylococcus aureus, Bacillus subtilis, Gram-negative bacteria: Escherichia coli, and fungal strains: Candida albicans and Aspergillus niger by tube dilution method. The compounds were also evaluated for antioxidant potential using DPPH free radical assay. Results of antimicrobial screening indicated that compound 1 (MICCA = MICAN= 0.10 µM/ml) was found to be most active antimicrobial agent comparable to standard drugs taken as well as showed good antioxidant potential (IC50=49.38µg/ml). The synthesized derivatives (1-14) were evaluated in vitro for their urease inhibitory activities by Indophenol method; most of the investigated compounds were potent inhibitors of Jack Bean Urease. The compounds 13 and 1 were found to be more potent urease inhibitors than the standard thiourea, depicting the IC50 values of 0.07 and 0.08 µM/ml, respectively (IC50 of thiourea =0.61 µM/ml). © 2018 iGlobal Research and Publishing Foundation. All rights reserved.

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