TETRA-PRIMER ARMS-PCR AS AN EFFICIENT ALTERNATIVE FOR SNPS DETECTION IN MOLECULAR DIAGNOSTIC: A COMPARISON STUDY
Journal Title: European Journal of Pharmaceutical and Medical Research - Year 2019, Vol 6, Issue 11
Abstract
Objective: The aim of this research was to compare the efficiency of the tetra-primer amplification refractory mutation system–polymerase chain reaction (ARMS-PCR) with restriction enzyme for detection of SNP anomalies in breast cancer blood samples. Method: one hundred breast cancer patients were analyzed for SNP mutation using tetra-primer ARMS-PCR technique and restriction enzyme (Pst1), to identify allele distribution of LCN2 on exon one a SNP rs11556770 (G/T). In this case, a single nucleotide transforms the amino acid from Glutamine, Gln (CAG), to Histidine, His (CAT). Result: Both techniques, tetra-primer amplification refractory mutation system–polymerase chain reaction (ARMS–PCR) and restriction enzyme, yielded normal (non-pathogenic) genotype pattern for the same SNP of sets samples, wherein tetra-primer ARMS PCR was 444bp, 156b, While restriction enzyme (PstI) three band 261bp, 135bp, 48bp. Nevertheless, both results estimate a normal allele for both techniques. Conclusion: Tetra-primer arms PCR method attained high specificity and sensitivity, along with the fact that this technique is more affordable, it can therefore be concluded that this method is an efficient and convenient option for detection of SNP anomalies for diagnostic purposes.
Authors and Affiliations
Prof. Sabah Linjawi
Keywords
Related Articles
- EP ID EP674670
- DOI -
- Views 181
- Downloads 0
HEPATITIS B VACCINE RESPONSE AMONG HEALTH WORKER KHAMIS MUSHAIT GENERAL HOSPITAL, SAUDI ARABIA, 2019
Introduction: The health care provider is face every days challenging of getting infection with may disease one of the important vaccine preventable disease is the hepatitis B vaccine the employer clinic in the Khamis Mu...
TREATMENT OF PRESBYOPES (PSYCOLOGICAL AND OPTICAL CONSIDERATION)
This article highlights some psychological and physiological factors that may be considered in explaining presbyopia to a presbyope. It also reviews selected optical and clinical factors relevant to the clinician’s deter...
DENGUE FEVER INFECTION EVALUATION OF ANTI-DENGUE IGM OR IGG USING NUMEROUS RAPID DIAGNOSTIC TESTS (RDTS)
Dengue fever (DV) is a mosquito-borne tropical disease caused by one of four dengue viruses. It is necessary to test the commercial rapid diagnostic kits for DV infection. In this study, we evaluated the accuracy and pre...
ANTIDIARRHEAL ACTIVITY OF AQUEOUS LEAF EXTRACT OF SWERTIA CHIRATA IN RATS
The effect of the aqueous extract of Swertia Chirata hum against diarrhea at 25,50 and 100 mg/kg body weight was evaluated by using gastrointestinal transit, diarrhea and enteropooling induced by castor oil models in fem...
OCCURRENCE AND ANTIBIOTIC RESISTANT PHENOTYPES OF STAPHYLOCOCCUS AUREUS ISOLATED FROM SOME HOSPITAL ENVIRONMENTS IN NASARAWA TOWN, NASARAWA STATE, NIGERIA
This work was aimed at determining the occurrence and antibiogram of Staphylococcus aureus isolated from some hospital environments in Nasarawa town, Nasarawa State, Nigeria. Out of the 160 swab samples collected in this...