The ability of the BANA test to detect different levels of P. gingivalis, T. denticola and T. forsythia
Journal Title: Brazilian Oral Research - Year 2010, Vol 24, Issue 2
Abstract
The aim of this study was to evaluate the ability of the BANA Test to detect different levels of Porphyromonas gingivalis, Treponema denticola and Tannerella forsythia or their combinations in subgingival samples at the initial diagnosis and after periodontal therapy. Periodontal sites with probing depths between 5-7 mm and clinical attachment level between 5-10 mm, from 53 subjects with chronic periodontitis, were sampled in four periods: initial diagnosis (T0), immediately (T1), 45 (T2) and 60 days (T3) after scaling and root planing. BANA Test and Checkerboard DNA-DNA hybridization identified red complex species in the subgingival biofilm. In all experimental periods, the highest frequencies of score 2 (Checkerboard DNA-DNA hybridization) for P. gingivalis, T. denticola and T. forsythia were observed when strong enzymatic activity (BANA) was present (p < 0.01). The best agreement was observed at initial diagnosis. The BANA Test sensitivity was 95.54% (T0), 65.18% (T1), 65.22% (T2) and 50.26% (T3). The specificity values were 12.24% (T0), 57.38% (T1), 46.27% (T2) and 53.48% (T3). The BANA Test is more effective for the detection of red complex pathogens when the bacterial levels are high, i.e. in the initial diagnosis of chronic periodontitis.
Authors and Affiliations
José Alexandre de Andrade, Magda Feres, Luciene Cristina de Figueiredo, Sérgio Luiz Salvador, Sheila Cortelli
Effect of different photo-initiators and light curing units on degree of conversion of composites
The aim of this study was to evaluate: (i) the absorption of photo-initiators and emission spectra of light curing units (LCUs); and (ii) the degree of conversion (DC) of experimental composites formulated with different...
Influence of alcohol consumption on alveolar bone level associated with ligature-induced periodontitis in rats
Alcohol consumption is a risk indicator for periodontal disease. The purpose of this study was to morphometrically evaluate the influence of alcohol consumption on alveolar bone level associated with ligature-induced per...
Renewing a partnership to further research and improve oral health
Mesiodistal width and proximal enamel thickness of maxillary first bicuspids
This study aimed at evaluating measurements relative to the mesiodistal crown width and enamel thickness of maxillary first bicuspids. The sample consisted of 40 extracted sound bicuspids (20 right and 20 left), selected...
Juveniles versus adults: differences in PGE2 levels in the gingival crevicular fluid during orthodontic tooth movement
This study aimed to investigate age-related changes in the biosynthetic capacity of prostaglandin E2 (PGE2) in the gingival crevicular fluid (GCF) during one month of orthodontic treatment. Twenty-five juvenile subjects...