The ability of the BANA test to detect different levels of P. gingivalis, T. denticola and T. forsythia
Journal Title: Brazilian Oral Research - Year 2010, Vol 24, Issue 2
Abstract
The aim of this study was to evaluate the ability of the BANA Test to detect different levels of Porphyromonas gingivalis, Treponema denticola and Tannerella forsythia or their combinations in subgingival samples at the initial diagnosis and after periodontal therapy. Periodontal sites with probing depths between 5-7 mm and clinical attachment level between 5-10 mm, from 53 subjects with chronic periodontitis, were sampled in four periods: initial diagnosis (T0), immediately (T1), 45 (T2) and 60 days (T3) after scaling and root planing. BANA Test and Checkerboard DNA-DNA hybridization identified red complex species in the subgingival biofilm. In all experimental periods, the highest frequencies of score 2 (Checkerboard DNA-DNA hybridization) for P. gingivalis, T. denticola and T. forsythia were observed when strong enzymatic activity (BANA) was present (p < 0.01). The best agreement was observed at initial diagnosis. The BANA Test sensitivity was 95.54% (T0), 65.18% (T1), 65.22% (T2) and 50.26% (T3). The specificity values were 12.24% (T0), 57.38% (T1), 46.27% (T2) and 53.48% (T3). The BANA Test is more effective for the detection of red complex pathogens when the bacterial levels are high, i.e. in the initial diagnosis of chronic periodontitis.
Authors and Affiliations
José Alexandre de Andrade, Magda Feres, Luciene Cristina de Figueiredo, Sérgio Luiz Salvador, Sheila Cortelli
Crown dimensions and proximal enamel thickness of mandibular second bicuspids
To achieve proper recontouring of anterior and posterior teeth, to obtain optimal morphology during enamel stripping, it is important to be aware of dental anatomy. This study aimed at evaluating crown dimensions and pro...
Renewing a partnership to further research and improve oral health
Juveniles versus adults: differences in PGE2 levels in the gingival crevicular fluid during orthodontic tooth movement
This study aimed to investigate age-related changes in the biosynthetic capacity of prostaglandin E2 (PGE2) in the gingival crevicular fluid (GCF) during one month of orthodontic treatment. Twenty-five juvenile subjects...
Pre-heated dual-cured resin cements: analysis of the degree of conversion and ultimate tensile strength
This study evaluated the degree of conversion (DC) and ultimate tensile strength (UTS) of dual-cured resin cements heated to 50º C prior to and during polymerization. Disc- and hourglass-shaped specimens of Rely X ARC (R...
Mechanical properties of one and two-step fluoridated orthodontic resins submitted to different pH cycling regimes
The aim of this study is to assess the in vitro shear bond strength and adhesive remnant index (ARI) of one and two-step fluoridated orthodontic resins under conditions that simulate high cariogenic challenge. Edgewise...