THE ACTIVITY OF DHAR IN ARABIDOPSIS THALIANA UNDER SALT STRESS
Journal Title: Naukovyi visnyk Chernivetskoho universytetu. Biolohiia (Biolohichni systemy) - Year 2017, Vol 9, Issue 1
Abstract
Unfavorable environmental conditions are damaging for plants. One of the negative factors that decreases the yield of crops is salinization of soils, which causes salt stress in plants and, as a result, increased generation of reactive oxygen species (ROS) in the cells. Higher plants have an anti-oxidant defense system that comprises antioxidant enzymes, in particular dehydroascorbate reductase (DHAR), which plays an important role in regulating the redox state of ascorbate by reducing its oxidized form. It is known that DHAR is critical for protecting plants from drought, heat, excessive light, ozone and high concentrations of heavy metal ions. Still, the role of this enzyme in salt stress tolerance has not yet been clarified enough. Therefore, the aim of this work was to investigate the effect of short-term salt stress on the activity of DHAR in Arabidopsis thaliana plants. In this study we used 4.5-5 week-old A. thaliana plants of the Columbia 0 ecotype. The plants were grown at a constant temperature of +20°С and an illumination of 2000 Lux under a 16-hour photoperiod. For the stress treatment, the aboveground part of the plant was separated from the root system and the cutting place was immersed in liquid Murashige and Skoog medium supplemented with 50, 100 and 200 mM sodium chloride. The specimens were incubated in the light or in the dark for 4 and 8 hours. The activity of DHAR was determined spectrophotometrically. The obtained data showed that 4 hours of salt stress did not cause significant changes in the activity of DHAR in Arabidopsis leaves compared with controls. Increased salt exposure of up to 8 hours led to an increase in DHAR activity by 20-23% only by application of the highest salt concentration (200 mM). This effect was observed in both, light and dark treatment conditions. Thus, our data shows the development of the stress response and the induction of protective mechanisms in the plant cell requires a certain amount of time. The obtained data indicate that DHAR in A. thaliana plants is involved in the
Authors and Affiliations
І. М. БУЗДУГА, І. Г. ГАВРИЛЮК, І. І. ПАНЧУК
Keywords
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