THE ANATOMICAL INVESTIGATIONS OF AERIAL AND UNDERGROUND PARTS OF ROUGH COCKLEBUR (XANTHIUM STRUMARIUM L .) AND CHROMATOGRAPHIC ANALYSIS OF TANNIN FRACTION
Journal Title: Postępy Fitoterapii - Year 2016, Vol 17, Issue 1
Abstract
The present work is a continuation of research on species Xanthium strumarium L . In the previous sections describe its pharmacological properties, phytochemical analysis of phenolic compounds, sterols and triterpenes and analyze the content and composition of essential oil . The first time the raw material was carried out systematic anatomical studies stems, leaves and roots . It has been shown that in all the examined organs are secreted structure in the form of channels, vascular tissue and hair . In the present discharge channels . The stem was a small secretory channels that form two rings external and internal . The root canals are secreted in the cortex secondary . The results of analysis of tannins in the examined organs Xanthium strumarium L . are as follows: the highest percentage shows the herb 10 .54%, fruits 7 .14% and the lowest roots 3 .35% . TLC analysis allowed to identify the presence of phenolic acids: caffeic, gallic and ferulic in all the tested extracts, and in addition to identify the chlorogenic acid from extracts of the herb, fruit and roots following acid hydrolysis . HPLC analysis of tannin fractions after hydrolysis of extracts from the herb, fruit and roots revealed the presence of the following phenolic acids in the herb: gallic, caffeic, protocatechuic, syringic, chlorogenic, ferulic and vanilic; in fruits gallic, caffeic, protocatechuic, chlorogenic, ferulic and vanillic; and in the roots of gallic, caffeic, chlorogenic, ferulic and vanilic acids .
Authors and Affiliations
Tadeusz Wolski, Tomasz Baj, Katarzyna Wolska, Wanda Zwolan
Keywords
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