The Application Of PCR RAPD Technique In Determining Female Sex Of Kapulasan (Nepheleum Lapaceum Mutabile)
Journal Title: JERAMI Indonesian Journal of Crop Science - Year 2019, Vol 1, Issue 2
Abstract
Kapulasan is one of horticultural plants derived from tropical region that has significant economic value. One of the obstacles in cultivating the plant is that kapulasan has flowering type of androdioceus, i.e. the male and the female flowers are separated. Female flowers produce hermaphroditic flowers that can produce fruits while the male ones will not produce fruits. In the cultivation of the plant, it is very difficult to determine the female flowers if they are propagated by seeds. It takes up to 7 years to determine the nature of the flowering. One of the important steps that can be done is through a system of early detection using molecular marks. RAPD technique as a simple technique can be used to distinguish the characteristics. The results of the research show that of the 250 primers used, the OPM-09 primer produces female sex bands related to the controller of the female kapulasan plants of which the ribbon produced is 512 bp in size.
Authors and Affiliations
Edi Wirman
Keywords
Related Articles
- EP ID EP532314
- DOI 10.25077/jijcs.1.2.1-7.2019
- Views 47
- Downloads 0
Aluminium stress examination of Pasaman local brown rice genotypes in early phase
One of brown rice resistance is tolerant to Aluminium stress. The research was conducted in Seed Technology Laboratory and shade net house of Faculty of Agriculture, Andalas University form March to June 2017. The resear...
Effect of Different Seed Water Content and Storage Duration towards Seed Viability of Local Genotype Brown Rice Daro Merah
Most of rice seeds experience after ripening problem which then result in seed dormancy. This kind of dormancy could be broken through various types of treatments, including storaging in dry place for certain period. Thi...
Isolation of Cellulose Degradation Bacteria (CDB) from acid soil as a potential candidate of organic waste degradation
Background: The study aimed to obtain CDB with high degraded activities, determined growth curve, protein content, and cellulase maximum activity (exoglucanase and endoglucanase). Method: The cellulose activity cal...
The Effect of Pollination Models on Yield of Red Pitaya (Hylocereus polyrhizus)
Red pitaya is getting more popular in Indonesia recently as one of high antioxidant sources with a promising economic value. However, the production of this CAM plant is restricted by low success percentage of its natura...
Effect Of Seed Hydration-Dehydration Integrated With Rhizobacteria On Viability And Vigor Of Deteriorated Soybean Seed
Soybeans undergo rapid deterioration due to its chemical composition and unfavourable storage conditions. The objective of the research was to determine the length period of seed hidration and dehydration which is integr...