The application of peptide sequencing for characterization of cetrorelix and its related impurities
Journal Title: World Journal of Pharmaceutical Sciences - Year 2016, Vol 4, Issue 8
Abstract
Electrospray ionization mass spectrometry (ESI-MS) was used to evaluate the structure of cetrorelix and the related impurities generated throughout the solid phase peptide synthesis (SPPS) reactions by Fmoc strategy. It was concluded that these by-products are formed as a result of incomplete removal of the protecting groups placed on side chains during synthesis. The impurity with 56 Da mass difference from cetrorelix was determined to be the result of failure in deprotection of L-serine residue and the impurity at 252 Da higher m/z than cetrorelix was due to the incomplete removal of Pbf from L-arginine. Peptide sequencing using ESI-MS allowed for rapid and reliable identification and characterization of the target peptide and its impurities. Furthermore, the information obtained from this method can offer a significant advantage for purity assessment of synthetic peptides which are used as therapeutic or research tools and it can also contribute a significant amount toward designing more efficient synthesis pathways for these molecules.
Authors and Affiliations
Dr. Shirin Hooshfar
Keywords
Related Articles
- EP ID EP199970
- DOI -
- Views 93
- Downloads 0
Phytochemical screening, antioxidant and antimicrobial activities of seeds of Foeniculum vulgare (ensilal)
Fennel (Foeniculum vulgare Mill) is one of the oldest spice plants which are considered as one of the world’s most important medicinal plants. Phytochemical screening, the total phenolic contents (TPC), total flavonoid c...
Histological Assessment and Quantification of Hypervitaminosis A-induced Fibrosis in Liver, Kidney and Testis of Albino Rats
The purpose of this work is to clarify if hypervitaminosis-A may induce fibrosis, then quantification and grading of this possible induced fibrosis. The present study was included two groups of male rats. Group I: con...
Method Development and Determination of Levodopa in Pharmaceutical Dosage Forms by Visible Spectrophotometry
Two simple, accurate and economic spectrophotometric methods A and B have been developed for the determination of levodopa in bulk and dosage forms. These methods are based on the formation of chloroform soluble ion-asso...
The effect of Ahl-1 recombinant lactonase on the resistance of Pseudomonas aeruginosa clinical isolates
Quorum quenching has lately become a focal point in research as a method of controlling infection and reducing virulence in pathogenic bacteria like P. aeruginosa. To investigate whether the ability of quorum quenching l...
Hospital Infection Control:Intensive Care Unit as an indicator
Introduction: The Hospital Infection Control Committee (HICC) has an arduous role in a tertiary care hospital where lapses in infection control measures can adversely impact patient morbidity and survival. Outbreaks of e...