The application of peptide sequencing for characterization of cetrorelix and its related impurities
Journal Title: World Journal of Pharmaceutical Sciences - Year 2016, Vol 4, Issue 8
Abstract
Electrospray ionization mass spectrometry (ESI-MS) was used to evaluate the structure of cetrorelix and the related impurities generated throughout the solid phase peptide synthesis (SPPS) reactions by Fmoc strategy. It was concluded that these by-products are formed as a result of incomplete removal of the protecting groups placed on side chains during synthesis. The impurity with 56 Da mass difference from cetrorelix was determined to be the result of failure in deprotection of L-serine residue and the impurity at 252 Da higher m/z than cetrorelix was due to the incomplete removal of Pbf from L-arginine. Peptide sequencing using ESI-MS allowed for rapid and reliable identification and characterization of the target peptide and its impurities. Furthermore, the information obtained from this method can offer a significant advantage for purity assessment of synthetic peptides which are used as therapeutic or research tools and it can also contribute a significant amount toward designing more efficient synthesis pathways for these molecules.
Authors and Affiliations
Dr. Shirin Hooshfar
Keywords
Related Articles
- EP ID EP199970
- DOI -
- Views 98
- Downloads 0
Preferred learning styles of second year medical students
Medical students differ in intelligence, personality, aptitudes and rate of learning. By identifying and adopting their preferred learning styles, the process of learning will be enhanced which further helps in achieving...
An observatory study about Diabetic Screening and Counseling OPD
Diabetes is one of the most costly and burdensome chronic diseases of contempraroy era. Individuals with type 2 diabetes are also at a significantly higher risk for coronary heart disease, peripheral vascular disease, hy...
Analytical Method Development and Validation of Abacavir in Pure and Pharmaceutical Dosage Forms by Using UV- Spectrophotometric Method
A novel, simple, accurate and precise Zero order derivative spectroscopic method was developed and validated for the estimation of Abacavir in bulk and Pharmaceutical dosage forms and has an absorption maximum at 296 nm...
A study of rheumatoid arthritis management with the triple combination of methotrexate + hydroxychloroquine + sulfasalazine and double combination of methotrexate + hydroxychloroquine
Rheumatoid arthritis is chronic multisystem disease, failure rate with monotherapy is high. The present study was designed to compare the efficacy and safety of triple combination of Methotrexate + Hydroxychloroquine + S...
Development and characterization of Nicorandil loaded maltodextrin based proniosomes
The aim of the study was to develop and characterize Nicorandil proniosomes by slurry method using the non-ionic surfactants like Span60, Brij72 and cholesterol as vesicle forming agents and maltodextrin as a carrier. Th...