The Beneficial Effect of Hydro-alcoholic Extract of Punica Granatum L. Leaves & Flower on Ethylene Glycol-Induced Kidney Calculi in Rats
Journal Title: Journal of Herbal Drugs - Year 2016, Vol 7, Issue 1
Abstract
Background & Aim: Punica granatum L. (pomegranate) is a widely used plant that has high nutritional value. This study evaluated prevention effect of hydro alcoholic extract of Punica granatum flower and leaves ethylene glycol-induced Nephrolithiasis in Wistar rats. Experimental: 20 male Wistar rats were randomly divided into 4 equal groups. Group I served as positive control and received distilled water for 28 days. Group II to IV received 1% (V/V) ethylene glycol (EG) in distilled water for 28 days. Group II served as EG or negative control. Group III and IV (preventive groups) received hydroalceholic extract of Punica granatum flower asand leaves orally for 28 days in dose of 400 mg/kg respectively. 24- hour urine samples were collected on day 0 and 28. Urine volume and urine oxalate levels were measured. On day 28, blood was collected for biochemical parameters (craetinine, urea and uric acid). Animals were sacrificed and kidneys were harvested, histopathologically evaluated for calcium oxalate (CaOx) crystals. Results: EG significantly increased urine oxalate and calcium, serum creatinine, urea and uric acid levels. It also significantly decreased urine citrate level in group III and IV resulted in significantly lower levels of urine oxalate and serum creatinine, urea and uric acid as compared to Group II (P < 0.001). Recommended applications/industrie: Hydro alcoholic extracts of Punica granatum flowers and leaves are effective in prevention of EGinduced nephrolithiasis in Wistar rats.
Authors and Affiliations
Shahram Pararin; Leila Rouhi; Abdollah Ghasemi Pirbalouti
Keywords
Related Articles
- EP ID EP23507
- DOI -
- Views 326
- Downloads 19
The Beneficial Effect of Hydro-alcoholic Extract of Punica Granatum L. Leaves & Flower on Ethylene Glycol-Induced Kidney Calculi in Rats
Background & Aim: Punica granatum L. (pomegranate) is a widely used plant that has high nutritional value. This study evaluated prevention effect of hydro alcoholic extract of Punica granatum flower and leaves ethylene...
Rapid screening of antioxidant activity, fracture rate and scavenging of free radicals by hairy root of Periwinkle (Catharanthus roseus L. G. Don)
Background & Aim: Oxidative stresses caused by free radicals are known to promote many diseases such as inflammation and cancer; therefore, any plant product that can inhibit free radical production can play an importa...
Phytochemical characteristics variation of different populations of Tanacetum parthenium cultivated in Shahrekord climatic
Background and aim: Tanacetum parthenium L. is herbaceous perennial medicinal plant belonging to the Asteraceae family. Feverfew is used as an analgesic, housing, fever-lowering, anti-rheumatism and migraines in traditio...
Evaluation of ecotype and chemotype diversity of Thymus daenensis Celak. on Isfahan and Chaharmahal va Bakhtiari provinces
Background & Aim: Thymus daenensis Celak. is one of the herbal and perennial medicinal plants belonging to Lamiaceae which grows widely in Isfahan and Chaharmahal va Bakhtiari provinces. All of its floral on aerial part...
Quantitative and qualitative changes of essential oil of Artemisia haussckenechtii in different phenological stages
Background & Aim: Artemisia species with common Persian name of Dermaneh are found all over Iran and are used for treatment in infectious diseases such as malaria, hepatitis and other diseases. Some Artemisia species are...