The contribution of proteins with binding activity and specific metabolic pathways in tolerating abiotic stress by canola: An in silico study
Journal Title: Journal of BioScience and Biotechnology - Year 2016, Vol 5, Issue 3
Abstract
Plants indicate different degrees of sensitivity and of tolerance upon encountering stressful conditions. In molecular level, the plant response is in a complex manner to such conditions. Obviously, the tolerant plants employ the molecular mechanisms that enable them to be tolerance/resistance. In order to investigate molecular mechanisms involved in the response of canola to stress conditions, two cultivars of canola including Sarigol (as a sensitive cultivar) and Hyola308 (as a tolerant cultivar) were in silico studied based on the differentially expressed proteins under abiotic stresses. The results indicated that in Hyola308, the overrepresented genes/proteins are mostly involved in response to stress, with the function of binding activity. In the case of Sarigol, the number of the genes/proteins involved in response to stress was low, while the most of its induced genes/proteins had the catalytic and antioxidant activities. Of 34 metabolic pathways, 12 pathways were common between the two cultivars, whereas numbers 17 and 5 were exclusive for Sarigol and Hyola308, respectively. Considering the tolerance of Hyola308, those unique metabolic pathways, including (1) protein processing in endoplasmic reticulum, (2) carbon fixation in photosynthetic organisms, (3) endocytosis, (4) spliceosome, and (5) fructose and mannose metabolism, could be in relation to high tolerance. PPI network illustrated interactions across differentially expressed proteins, in which 8 nodes for Sarigol and 5 nodes for Hyola308 showed high interactions, called “hub” nodes. The genes UGD2, TPI, and AT3G09440 are introduced as potential candidates to be regarded in genetic engineering of canola due to their core central roles in PPI network. In conclusion, Sarigol and Hyola308 represented some similarity and differences at the molecular level in responding to abiotic stresses, in which unique mechanisms represented by Hyola308 might be a key in tolerating abiotic stresses because of its high tolerance to these kinds of stresses.
Authors and Affiliations
Reza Shokri Gharelo, Ali Bandehagh
Keywords
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